5x HOT FIREPol Blend Master Mix Ready to Load
Ready to load hot-start PCR master mix for more demanding PCR assays
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Really useful product
PCR amplification
Using it for a long time. Really like the Master mix. Easy to use and saves time.
Review Date: 24 Nov 2021 | Solis BioDyne
Awesome product.
Gene amplification (PCR)
The easiest and most convenient way to amplify your genes. No mess or making errors while making your own master mix. Ready to use and saves time.
Review Date: 8 Feb 2021 | Solis BioDyne
Excellent enzyme, recommended.
Perform PCR of fragments up to 3Kb
Great results obtained. Good value for money, hotstart is useful. We use this almost exclusively for our qPCR and endpoint PCR.
Review Date: 24 Jul 2020 | Solis BioDyne
5x HOT FIREPol® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water), additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) that allow to monitor progress during electrophoresis.
5x HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to regular Taq polymerase.
In addition, the mix also allows amplification of longer fragments.
Generated PCR products are compatible with blunt-end and T/A cloning procedures.
HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to regular Taq polymerase.
- increased yield, sensitivity and specificity
- up to 5x higher fidelity
- suitable for templates up to 5 kb
- reduced primer dimer formation
- load directly on gel after PCR
