Cod UNG

ArcticZymes are the inventors of Cod Uracil-DNA Glycosylase (Cod UNG). Developed from Atlantic Cod it is the only commercially available UNG enzyme that is completely and irreversibly inactivated by moderate heat treatment. The enzyme is produced in a recombinant E. coli (ung-) strain that contains a modified Cod UNG gene.

There are several commercially available Uracil-DNA glycosylases on the market today. Most of them are of bacterial origin and work well if you have no intention to further analyze the PCR products post-PCR. However, if you want to store your PCR products for downstream analysis such as cloning and sequencing, the reactivation of UNG and subsequent degradation of your PCR products are a problem with most of the commercially available UNGs. Cod UNG from ArcticZymes is completely and irreversibly inactivated by heat thus ensuring that sample integrity is maintained long-term regardless of storage conditions.

Key Features:

• Heat-labile - Completely and irreversibly inactivated at 55°C
• Contamination control - ideal in applications below
• Use of Cod UNG makes contamination control possible in RT-PCR
• Does not degrade PCR product post-PCR. This makes downstream use of the PCR product possible
• High purity enzyme, tested free of contaminating nucleases
• Detergent free
• Post-PCR Analysis - Enables post-PCR analysis
• Available in Glycerol-free and Triton-Free

Applications

• Ideal for contamination control in PCR carry-over prevention
• RT-PCR
• RT-qPCR
• qPCR
• kPCR
• RT-LAMP
• Heat-labile Uracil-DNA Glycosylase