Endonucleases Non-Specific, HL-SAN
HL-SAN efficiently removes nucleic acids from buffers in protein purification, excelling in high-salt conditions to reduce aggregation. Ideal for removing nucleic acids from proteins with high DNA/RNA affinity, it performs well during lysis, early purification stages, and high-salt eluates. This cold-adapted enzyme works effectively at ambient temperatures and during overnight digestion at 4°C. Triton free available.
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HL-SAN efficiently removes nucleic acids from buffers typically used in protein purification. Due to its high salt tolerance, it is the obvious choice for host-cell DNA removal in settings where salt is added to reduce aggregation. Especially efficient for removing nucleic acids from proteins with high affinity for DNA and RNA. Proven performance during lysis and early stages of protein purification processes, as well as high-salt eluates. Cold-adapted enzyme with excellent performance also at ambient temperatures and during over-night digestion at 4°C.
Key advantages with this Salt Active Nuclease:
- Non specific endonuclease
- Optimum activity at high salt concentration (0.5 M NaCl)
- Active at low temperatures (20% at 6ºC)
- Easily inactivated
- Broad pH range
- Temperature stable
- Triton free available
Recommended Applications:
- Complete removal of DNA
- Viscosity reduction
