Isothermal Low Temperature Range
ArcticZymes' IsoPol® isothermal polymerases are engineered for rapid, efficient DNA amplification in point-of-care (POC) settings. With enhanced strand displacement, high processivity, and tolerance to biological matrices, they allow faster diagnostics with minimal sample prep. IsoPol BST+ and IsoPol SD+ excel in challenging environments, supporting popular methods like LAMP for quick, reliable results at optimal temperatures.
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To accommodate the need for high quality enzymes for isothermal amplification ArcticZymes developed the IsoPol® series of polymerases for rapid, efficient DNA amplification in point-of-care (POC) settings. With enhanced strand displacement, high processivity, and tolerance to biological matrices, they allow faster diagnostics with minimal sample prep. IsoPol BST+ and IsoPol SD+ excel in challenging environments, supporting popular methods like LAMP for quick, reliable results at optimal temperatures.
Isothermal Low Temperature Range:
For isothermal amplification at lower temperatures (37°C and below) we offer two enzymes, IsoPol® DNA Polymerase and IsoPol® SD+. Both enzymes exhibit excellent processivity and high strand displacement activity, having 5’-3’ polymerase activity while lacking both 3’-5’and 5’-3’ exonuclease activity.
IsoPol SD+ and IsoPol DNA Polymerase are ideal for amplification at temperatures above 20°C and 37°C.
IsoPol® SD+ is an engineered version of IsoPol® DNA Polymerase with even stronger strand displacement and higher salt tolerance.
Isothermal Low Temperature Range:
IsoPol® BST+ is a heat-tolerant BST polymerase (large fragment) with enhanced strand-displacement activity. IsoPol® BST+ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.
Key features
- IsoPol® BST+ lacks 5’-3’- and 3’-5’-exonuclease activity.
- Inactivation - IsoPol® BST+ is completely inactivated after one minute incubation at 95°C
- Enhanced strand displacement and processivity.
- Increased salt tolerance.
- Available Glyrcerol-Free
We offer a unique family of DNA polymerases selected for isothermal amplification, originating from a Marine bacterium, expressed in E. coli and soil, hot springs, ocean sediment.
IsoPol BST+ is an in silico designed homologue of Bst DNA Polymerase (large fragment) suitable for amplification at elevated temperatures with an optimum at 65°C.
IsoPol BST+ and IsoPol SD+ are engineered for enhanced amplification performance and higher inhibitor tolerance.
