EZ-Magna ChIRP RNA Interactome Kit
Allows analysis and mapping of regions of chromatin interacting with chromatin-associated RNAs.
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EZ- Magna ChIRP RNA Interactome Kit -Isolation and characterization of non-coding RNA:chromatin complexes.
The Magna ChIRP RNA Interactome Kit uses the ChIRP method (Chromatin Isolation by RNA Purification) to enable identification and analysis of regions of chromatin that interact with a particular chromatin-associated RNA. This novel anti-sense probe-based capture strategy uses cross-linked chromatin to allow unbiased discovery of RNA-associated DNA sequences and proteins.
To perform ChIRP two pools consisting of mulitple biotinylated oligonucleotide probes complementary to the RNA of interest are used. These probe sets are combined with chromatin and hybridized to the chromatin-associated RNA. Complexes containing biotinylated-probes bound to the chromatin-associated RNA are then isolated using streptavidin magnetic beads. DNA or RNA can then be recovered and analyzed by quantitative PCR or next generation sequencing (ChIRP-seq). Alternatively, proteomic analysis of the recovered chromatin can be performed.
Features:
- Recover non-coding RNA containing complexes using lncRNA and other types of chromatin associated RNA as targets
- Identify sites of genomic interaction of chromatin associated RNA
- Perform proteomic analysis of chromatin associated RNA regulatory complexes
- Includes PureProteome™ Streptavidin magnetic beads, plus all key buffers, enzymes, and reagents
- All kits include a negative control probe set and a detailed protocol
- EZ-Magna ChIRP kit includes a biotinylated TERC lncRNA positive control probe set and qPCR and RT-qPCR detection primer sets.
- Biotinylated capture probe sets for lncRNA TERC and NEAT1 sets are also available separately
Applications:
- Purification with magnetic beads
- DNA-binding Proteins
- RNA Detection
- DNA Sequencing
- Protein Determination
- Protein Interaction Assays
