Fluorescein-12-dCTP
Fluorescein-12-dCTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation.
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Great results, but would like more customer support.
Analyze RT and PCR
Product is definitely better than other products available in market, they are more stable than indicated on the product. I have seen lot to lot variability sometimes, but it's hard to show that because of other changes in experiments. Product lead time is always a concern. Never got an opportunity to collaborate for product development, which is why I had to work with another company for a CLIA product I developed.
Review Date: 7 Dec 2020 | Jena Bioscience
Fluorescein-12-dCTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine.
Recommended Fluorescein-12-dCTP/dCTP ratio for PCR and Nick Translation: 30-50% Fluorescein-12-dCTP-/ 70-50% dCTP
Please note: Protect the Dye-labeled dCTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dCTP/dCTP ratio is recommended.
