StemSpan™ CD34+ Expansion Supplement (10X)
Serum-free culture supplement for expansion of human CD34+ hematopoietic cells
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Great product quality
We use it for CD34+ primary cell expansion
The product is very good and gives consistent results as it has been calibrated with all the supplements otherwise needed to be added individually. Very good for repetitiveness.
Review Date: 25 Jul 2024 | STEMCELL Technologies Inc.
StemSpan™ CD34+ Expansion Supplement typically promotes ~40-fold expansion of total nucleated cells in 7-day liquid cultures of CD34+ human cord blood (CB) cells. After one week, ~40% of the cultured cells express CD34, indicative of >10-fold expansion of input CD34+ CB cells. See data tab for more details. StemSpan™ CD34+ Expansion Supplement (10X) contains a combination of recombinant human cytokines (Flt3L, SCF, IL-3, IL-6 and TPO) and other additives formulated to selectively promote the expansion of CD34+ cells isolated from human CB and bone marrow (BM) samples. The CD34+ Expansion Supplement is intended for use in combination with StemSpan™ SFEM, SFEM II and -ACF serum-free expansion media, or any other media for culturing human hematopoietic cells.
When added to serum-free medium, the CD34+ Expansion Supplement typically promotes ~40-fold expansion of total nucleated cells in 7-day liquid cultures of CD34+ human CB cells. After one week, ~40% of the cultured cells express CD34, indicative of 10-fold expansion of input CD34+ CB cells. This supplement stimulates greater CD34+ cell expansion when compared to StemSpan™ CC100 and CC110. See data tab for more details.
