ZymoBIOMICS™ DNA/RNA Miniprep Kit
Purification of microbial DNA/RNA for microbiome or metagenome analyses
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Bacterial communities from plastic debris
The kit is easy to use because the protocol is well described. You can obtain both DNA and RNA with good quality from the same sample.
Review Date: 12 May 2023 | Zymo Research
RNA extraction for coral fragments
The quality of the RNA obtained was good but the quantity was not great. It is not a great option if you have to process hundreds of samples and don’t have a liquid handler. However for a small amount of samples and the proper magnet it would be a great alternative as it comes with PK and dnase steps incorporated
Review Date: 6 Dec 2022 | Zymo Research
Genomic DNA extracted from mice fecal samples
The ZymoBIOMICS DNA MiniPrep Kit is a very easy-to-use kit, with very clear and straightforward instructions, and it provides high-quality results. Particularly for our research, we extracted DNA from mice fecal samples with high precision, which was later used for sequencing, with accurate results. We were worried since some of the mice were fed a highl-fat diet, but this did not impact the DNA quality nor the sequencing results. Besides, compared to other DNA extraction kits, the Zymo MiniPrep kit includes an additional filter that I have not previously seen in other products, which I believe makes the difference in obtaining very pure DNA. For example, most samples were 260/280 > 2.0, and 260/230 between 1.9 and 2.10.
Review Date: 22 Nov 2022 | Zymo Research
gene expression
It works well with easy operation
Review Date: 17 Jun 2022 | Zymo Research
extract DNA & RNA from microbial communities
This kit worked well for my samples (stored in DNA/RNA Shield) and was fairly easy to use. I liked that the spin filters are color-coded to help you keep track of which is DNA and which is RNA. The only downside was that the protocol was a little hard to follow. You have to flip back and forth between the main protocol and the appendix during the DNase treatment step. Otherwise, it's a great kit.
Review Date: 25 Jan 2022 | Zymo Research
Microbiology
I really like the kit. It is easy to use and provides high yield and high quality DNA and RNA.
Review Date: 24 Nov 2021 | Zymo Research
Clinical metagenomics
This kit consistently facilitates extraction of high yield and high quality DNA.
Review Date: 15 Jun 2021 | Zymo Research
Environmental microbiology
This DNA extraction kit has proved to be the best one I've used to obtain DNA from low biomass samples I work with. My only issue with it is the amount of changes of collection tubes needed, which I hope gets optimized later on. But overall I am very happy with this product.
Review Date: 15 Jun 2021 | Zymo Research
Biochemistry research
Product inlay is well written and makes the product very easy to use.
Review Date: 25 May 2021 | Zymo Research
Diagnostics
I love the versatility of the kit. You're able to isolate all nucleic acid, either combined or separated into RNA and DNA. It's like having 3 kits in one!
Review Date: 25 May 2021 | Zymo Research
The ZymoBIOMICS® DNA/RNA Miniprep Kit is designed for purifying DNA and RNA from a wide array of sample inputs (e.g. feces, soil, plant, water, and biofilms) that is ready for microbiome or metagenome analyses. The ZymoBIOMICS® innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The provided DNA/RNA Shield™ preserves nucleic acids at ambient temperatures, providing an unbiased molecular snapshot of the sample. The procedure uses Zymo-Spin™ Column technology that results in high-quality DNA and total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors (e.g. polyphenols, humic acids, and fulvic acids). Ready for RT-PCR, arrays, sequencing, etc.