"Finnzymes' unique Phusion™ High-Fidelity DNA Polymerase offers performance that no other enzyme can match. The Phusion DNA Polymerase generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. The unique structure and characteristics of Phusion DNA Polymerase make it a superior choice for cloning and set a new standard for PCR performance. The error rate…
DyNAmo™ Probe qPCR Kit is a ready-to-use 2x master mix for highly specific quantitative PCR. The master mix contains all reagents needed for qPCR. Only template DNA, PCR primers and probe need to be added by the user.The 2x master mix includes the efficient hot start Thermus brockianus DNA polymerase. ROX™ passive reference dye is also supplied with the kit in a separate tube for instruments that require ROX normalization. DyN…
Phusion™ RT-PCR Kit is a complete two-step kit designed for high-fidelity RT-PCR. The kit contains the full set of reagents required for performing cDNA synthesis and PCR in two steps. In the first step, a variety of RNA templates and all priming options may be used for efficient synthesis of cDNA with M-MuLV Reverse Transcriptase.In the second step, Phusion Hot Start High-Fidelity DNA Polymerase is used for cDNA amplification…
Phusion™ Flash High-Fidelity PCR Master Mix is a 2x master mix enabling the use of extremely short PCR protocols without compromising neither the fidelity nor the yield in the reaction. Phusion Flash DNA Polymerase used in the master mix is a proofreading DNA polymerase modified from Phusion Hot Start High-Fidelity DNA Polymerase. Phusion Flash High-Fidelity PCR Master Mix enables the use of extremely short cycling protocols w…
Finnzymes’ DyNAmo™ Flash SYBR® Green qPCR Kit is developed for fast real-time qPCR. It provides qPCR results faster than other SYBR Green kits without compromising the qPCR performance.A DNA-binding domain attached to the polymerase in this kit improves the physical stability of the polymerase-DNA complex, thus enhancing the processivity and robustness of the amplification. The high amplification efficiency of DyNAmo Flash SYB…
The DyNAmo™ cDNA Synthesis Kit is intended for cDNA synthesis for two-step quantitative reverse transcription-PCR (qRT-PCR) applications. This kit can be used in conjunction with Finnzymes' DyNAmo qPCR Kits or with any other qPCR kit suitable for the application.The DyNAmo cDNA Synthesis Kit includes all necessary reagents for cDNA synthesis to be used in qPCR. Either total RNA, messenger RNA, viral RNA or in vitro transcribed…
The Phusion™ Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with Quick T4 DNA Ligase. The resulting…
Phusion™ High-Fidelity DNA Polymerase offers accuracy (error rate 4.4 x 10-7) and speed previously unattainable with a single enzyme, even on your most difficult templates. Phusion™ High-Fidelity PCR Kit contains all the necessary reagents for performing 50 amplifications in 50 μl volume or 125 amplifications in 20 μl volume. The kit also contains control template (λ DNA) and two primer sets (1.3 kb and 10 kb) for performing 2…
Finnzymes' DyNAzyme™ II Hot Start DNA Polymerase is a chemically modified hot start form of the DyNAzyme™ II DNA Polymerase originating from Thermus brockianus. DyNAzyme II Hot Start DNA Polymerase is inactive at room temperature, and the activity is recovered by a 10-minute incubation step at 94 °C.The enzyme improves the specificity of PCR by preventing the extension of non-specifically bound primers during reaction setup an…
DyNAzyme™ II PCR Master Mix is available as a 2x mix containing recombinant DyNAzyme II DNA Polymerase (isolated and purified from E.coli strain carrying a plasmid with the cloned DyNAzyme™ DNA Polymerase gene from Thermus brockianus), nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added by the user.
