The unique Phusion™ High-Fidelity DNA Polymerase with a dsDNA-binding domain is now available with a hot start modification. The new Affibody®-based inactivation method of Phusion DNA Polymerase increases the specificity of PCR amplification by preventing non-specific extension of DNA substrate at ambient temperatures prior to the first cycle of PCR.In addition to the extreme fidelity, speed and robustness, Phusion Hot Start…
"Finnzymes' unique Phusion™ High-Fidelity DNA Polymerase offers performance that no other enzyme can match. The Phusion DNA Polymerase generates PCR products with accuracy and speed previously unattainable with a single enzyme, even on your most difficult templates. The unique structure and characteristics of Phusion DNA Polymerase make it a superior choice for cloning and set a new standard for PCR performance. The error rate…
DyNAmo™ Probe qPCR Kit is a ready-to-use 2x master mix for highly specific quantitative PCR. The master mix contains all reagents needed for qPCR. Only template DNA, PCR primers and probe need to be added by the user.The 2x master mix includes the efficient hot start Thermus brockianus DNA polymerase. ROX™ passive reference dye is also supplied with the kit in a separate tube for instruments that require ROX normalization. DyN…
Phusion™ RT-PCR Kit is a complete two-step kit designed for high-fidelity RT-PCR. The kit contains the full set of reagents required for performing cDNA synthesis and PCR in two steps. In the first step, a variety of RNA templates and all priming options may be used for efficient synthesis of cDNA with M-MuLV Reverse Transcriptase.In the second step, Phusion Hot Start High-Fidelity DNA Polymerase is used for cDNA amplification…
Phusion™ Flash High-Fidelity PCR Master Mix is a 2x master mix enabling the use of extremely short PCR protocols without compromising neither the fidelity nor the yield in the reaction. Phusion Flash DNA Polymerase used in the master mix is a proofreading DNA polymerase modified from Phusion Hot Start High-Fidelity DNA Polymerase. Phusion Flash High-Fidelity PCR Master Mix enables the use of extremely short cycling protocols w…
Finnzymes’ DyNAmo™ Flash SYBR® Green qPCR Kit is developed for fast real-time qPCR. It provides qPCR results faster than other SYBR Green kits without compromising the qPCR performance.A DNA-binding domain attached to the polymerase in this kit improves the physical stability of the polymerase-DNA complex, thus enhancing the processivity and robustness of the amplification. The high amplification efficiency of DyNAmo Flash SYB…
The DyNAmo™ cDNA Synthesis Kit is intended for cDNA synthesis for two-step quantitative reverse transcription-PCR (qRT-PCR) applications. This kit can be used in conjunction with Finnzymes' DyNAmo qPCR Kits or with any other qPCR kit suitable for the application.The DyNAmo cDNA Synthesis Kit includes all necessary reagents for cDNA synthesis to be used in qPCR. Either total RNA, messenger RNA, viral RNA or in vitro transcribed…
The Phusion™ Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with Quick T4 DNA Ligase. The resulting…
Phusion™ High-Fidelity DNA Polymerase offers accuracy (error rate 4.4 x 10-7) and speed previously unattainable with a single enzyme, even on your most difficult templates. Phusion™ High-Fidelity PCR Kit contains all the necessary reagents for performing 50 amplifications in 50 μl volume or 125 amplifications in 20 μl volume. The kit also contains control template (λ DNA) and two primer sets (1.3 kb and 10 kb) for performing 2…
Thermostable DyNAzyme™ I DNA Polymerase is isolated and purified from Thermus brockianus, which is a Finnzymes proprietary strain. DyNAzyme I DNA Polymerase is more thermostable and tolerates DMSO better than Taq based polymerases.DyNAzyme I DNA Polymerase has a half-life of 3 hours at 96 °C. DyNAzyme I DNA Polymerase contains 5’-->3’ exconuclease activity but lacks 3'-->5' proofreading activity. DyNAzyme I DNA Polymerase is p…
Thermostable DyNAzyme™ II DNA Polymerase is isolated and purified from E.coli strain carrying a plasmid with the cloned DyNAzyme™ DNA Polymerase gene from Thermus brockianus which is a Finnzymes proprietary strain. DyNAzyme II DNA Polymerase is more thermostable and tolerates DMSO better than Taq based polymerases. DyNAzyme II DNA Polymerase has a half-life of 2.5 hours at 96 °C. DNA Polymerase contains 5’-->3’ exconuclease ac…
Finnzymes' DyNAzyme™ II Hot Start DNA Polymerase is a chemically modified hot start form of the DyNAzyme™ II DNA Polymerase originating from Thermus brockianus. DyNAzyme II Hot Start DNA Polymerase is inactive at room temperature, and the activity is recovered by a 10-minute incubation step at 94 °C.The enzyme improves the specificity of PCR by preventing the extension of non-specifically bound primers during reaction setup an…
DyNAzyme™ II PCR Master Mix is available as a 2x mix containing recombinant DyNAzyme II DNA Polymerase (isolated and purified from E.coli strain carrying a plasmid with the cloned DyNAzyme™ DNA Polymerase gene from Thermus brockianus), nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added by the user.
DyNAzyme™ EXT DNA Polymerase is an optimized mixture of DyNAzyme™ II DNA Polymerase and a proofreading enzyme. It is a versatile and easy-to-use enzyme especially suited for handling demanding reaction conditions without time-consuming optimization. DyNAzyme EXT Polymerease offers exceptional performance that Taq based mixtures cannot match.
DyNAzyme™ I DNA Polymerase Kit contains DyNAzyme™ I DNA Polymerase, buffers, ready-to-use DNA size standard (λ DNA Hind III and øX174 DNA Hae III digest) and nucleotides for 100 amplifications in 50 μl volume. The kit also contains control template and primers for 40 control reactions.
DyNAzyme™ EXT DNA Polymerase is an optimized mixture of DyNAzyme™ II DNA Polymerase and a proofreading enzyme. It is a versatile and easy-to-use enzyme especially suited for handling demanding reaction conditions without time-consuming optimization.DyNAzyme™ EXT PCR Kit is designed to provide superior PCR amplifications for 100 long and difficult PCR or 200 standard amplifications. The kit also contains control template and tw…
Phusion™ High-Fidelity DNA Polymerase offers accuracy (error rate 4.4 x 10-7 when using Phusion™ HF Buffer) and speed previously unattainable with a single enzyme, even on your most difficult templates. Phusion™ High-Fidelity PCR Master Mix with Phusion™ HF Buffer is a convenient 2x mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer (2x Phusion HF Buffer) including MgCl2 for 100 reactions in 50 μl…
Phusion™ High-Fidelity DNA Polymerase offers accuracy (error rate 4.4 x 10-7 when using Phusion™ HF Buffer) and speed previously unattainable with a single enzyme, even on your most difficult templates. However, Phusion™ GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, i.e. GC-rich templates or those with complex secondary structures. Use of the Phusion GC Buffer is recommend…
RobusT™ I RT-PCR Kit is a high-fidelity one-tube system that is designed for convenient, sensitive and reproducible amplification of a specific target RNA up to 6.0 kb in length. The performance and excellent thermostability of the RobusT I RT-PCR Kit is based on AMV Reverse Transcriptase (AMV RT) in the first strand cDNA synthesis and on DyNAzyme™ EXT DNA Polymerase in the second strand synthesis and subsequent DNA amplificat…
RobusT™ II RT-PCR Kit is a high-fidelity one-tube system that is designed for convenient, sensitive and reproducible amplification of a specific target RNA up to 6.0 kb in length. The performance and excellent thermostability of the RobusT II RT-PCR Kit is based on M-MuLV Reverse Transcriptase RNase H- in the first strand cDNA synthesis and on DyNAzyme™ EXT DNA Polymerase in the second strand synthesis and subsequent DNA ampli…
DyNAmo™ SYBR® Green qPCR Kit is a ready-to-use 2x master mix for sensitive, quantitative real-time PCR. Only template DNA and PCR primers need to be added by the user. DyNAmo™ DNA Polymerase (modified Thermus brockianus DNA polymerase) in this kit incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex. The kit is compatible with block-based real-time qPCR instruments that do…
DyNAmo™ SYBR® Green qPCR Kit with ROX™ passive reference dye is a ready-to-use 2x master mix for sensitive, quantitative real-time PCR. The ROX™ passive reference dye is supplied with the kit in a separate tube. Only template DNA and PCR primers need to be added by the user. DyNAmo™ DNA Polymerase (modified Thermus brockianus DNA polymerase) in this kit incorporates a non-specific DNA-binding domain that lends physical stabili…
DyNAmo™ HS SYBR® Green qPCR Kit is a ready-to-use 2x master mix for highly specific quantitative PCR. The 2x master mix includes a hot start version of a modified Thermus brockianus DNA polymerase. The modified DNA polymerase incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex. The kit is compatible with all block-based real-time qPCR instruments. ROX™ passive reference dy…
DyNAmo™ Capillary SYBR® Green qPCR Kit is a ready-to-use 2x master mix for highly specific quantitative PCR with instruments using capillary tubes. The 2x master mix includes a hot start version of a modified Thermus brockianus DNA polymerase. The modified DNA polymerase incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex.
DyNAmo™ SYBR® Green 2-step qRT-PCR Kit enables reproducible detection and quantification of RNA with great sensitivity and specificity. The kit contain all necessary reagents for cDNA synthesis from various RNA sources and for subsequent qPCR. Both random primers and oligo(dT) primers are included in the kit. Alternatively, the user can use gene specific primers. The reverse transcriptase in the kit is M-MuLV RNase H+, which p…
