Titan UHPLC columns, based on 1.9 µm totally porous monodisperse silica, outperform other UHPLC columns. These UHPLC columns provide the performance scientists expect (typically > 250,000 N/m), but at a greatly reduced cost. With the narrowest particle size distribution of all available sub-2 µm porous particles, Titan columns are the outcome of the highly efficient patent pending Ecoporous™ silica manufacturing process tha…
SP-2560 Capillary GC Column• Application: This highly polar biscyanopropyl column was specifically designed for detailed separation of geometricpositional (cis/trans) isomers of fatty acid methyl esters (FAMEs). It is extremely effective for FAME isomer applications.• USP Code: This column meets USP G5 requirements.• Phase: Non-bonded; poly(biscyanopropyl siloxane)• Temp. Limits: Subambient to 250 °C (isothermal or programmed
Ascentis Express RP-Amide HPLC columns are the most recent product additions to the Supelco HPLC product line. Combining an embedded polar group (EPG) stationary phase with the Fused-Core particles, Ascentis Express RP-Amide provides a host of useful benefits to the HPLC chromatographer. Ascentis Express RP-Amide is a blend of modern phase technology and innovative particle technology. The Fused-Core particle provides…
C18 HPLC column for High Speed and High Resolution Applications. Ascentis® Express C18 is the first choice for starting a new method. Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles at lower system pressure. With identical conditions, the Ascentis Express C18 column performs more than 4X faster tha…
Classic reversed-phases for all general-purpose applications. Features: Excellent peak shape and efficiency compared to competitive columns Classic reversed-phase retention and selectivity Excellent, no-bleed character for LC-MS applications C18 is generally more retentive than the C8 Column Details: Chemistry: C18 Particle Size: 5 µm Pore Size: 180 Å Length: 20, 30, 50, 100, 125, 150, 250 mm ID: 2.1, 3.0, 4.…
TruPAGE™ gels offer improved resolution, tear resistance, and an extended shelf-life over the currently available precast polyacrylamide gels. TruPAGE™ gels are formulated with Triethanolamine (TEA)-Tricine, featuring a neutral pH running environment. TruPAGE™ gel cassettes have a hassle-free design. Extra tall wells to prevent lane-to-lane overflow. No comb to remove, preventing torn or bent wells. Instead,…
Sufficient for 100 colorimetric or fluorometric tests
sufficient for 200 fluorometric tests
Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we h…
SLB®-IL111 Capillary GC Column for FAME and Petroleum Applications. World’s first commercial column to rate over 100 on our GC column polarity scale. Selectivity most orthogonal to non-polar and intermediate polar phases, resulting in very unique elution patterns. Maximum temperature of 270 ºC is very impressive for such an extremely polar column. Great choice for separation of polarizable analytes (contain double and/or tripl…
SLB-5ms Capillary GC Column Application: The 5% phenyl equivalent phase provides a boiling point elution order with a slight increase in selectivity, especially for aromatic compounds. The low bleed characteristics, inertness, and durable nature make it the column of choice for environmental analytes (such as semivolatiles, pesticides, PCBs, and herbicides) or anywhere a low bleed non-polar column is required. USP Code: Th…
BioReagent, suitable for electrophoresis, 0.1 % (w/v) in 5% acetic acid
Certified reference material, 20 μg/mL in methanol, ampule of 1 mL
Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein.
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase,…
BIOshell™ A160 Peptide C18, 2.7 μm HPLC Columns BIOshell A160 Peptide C18 columns packed with 2.7 μm particles, 160 Å pores, are as efficient as sub-2-μm porous particles, but at ½ the backpressure.These columns can be operated long term at pressures up to 600 bar (60 MPa or 8500 psi), temperatures up to 90 °C and within the pH range of 1 to 8. BIOshell A160 Peptide C18 columns can be run on all HPLC systems including UHPLC s…
SLB-IL60 Ionic Liquid Capillary GC ColumnApplication: Modified (deactivated) version of SLB-IL59 provides better inertness. Selectivity more polar than PEG/wax phases, resulting in unique elution patterns. Higher maximum temperature than PEG/wax columns (300 ºC compared to 270-280 ºC). Excellent alternative to existing PEG/wax columns. Also a good GCxGC column choice. Launched in 2012.USP Code: NonePhase: Non-bonded; 1,12-Di(t…
SLB-IL59 Ionic Liquid Capillary GC Column• Application: Selectivity more polar than PEG/wax phases, resulting in unique elution patterns. Higher maximum temperature than PEG/wax columns (300 ºC compared to 270-280 ºC). Great choice for analysis of neutral and moderately basic analytes. Launched in 2009.• USP Code: None• Phase: Non-bonded; 1,12-Di(tripropylphosphonium)dodecane bis(trifluoromethylsulfonyl)imide• Temp. Limits: Su…
SUPELCOWAX® 10 Capillary GC Column• Application: This column is based on one of the most widely used polar phases, Carbowax 20M, and is a polar column suitable for analyses of solvents, fatty acid methyl esters (FAMEs), food, flavor and fragrance compounds, alcohols, and aromatics. Additionally, this column is a great choice when a polar general purpose column is required.• USP Code: This column meets USP G16 requirements.• Ph…
Equity-5 Capillary GC Column• Application: This popular column is designed for general purpose applications where a non-polar column is required. The low phenyl content provides thermal stability compared to 100% poly(dimethyl siloxane) columns.• USP Code: This column meets USP G27 and G36 requirements.• Phase: Bonded; poly(5% diphenyl/95% dimethyl siloxane)• Temp. Limits: ≤0.32 mm I.D., • Temp. Limits: ≤0.32 mm I.D., ≥2 μm: -…
SPB-5 Capillary GC Column• Application: This non-polar general purpose column provides primarily a boiling point elution order with a slight increase in selectivity, especially for aromatic compounds.• USP Code: This column meets USP G27 and G36 requirements.• Phase: Bonded; poly(5% diphenyl/95% dimethyl siloxane)• Temp. Limits: ≤0.32 mm I.D., • Temp. Limits: ≤0.32 mm I.D., ≥2 μm: -60 °C to 300 °C (isothermal or programmed)• T…
The Perfect Combination of Low Bleed, Thermal Stability, and Easy Puncturability!Molded Thermogreen LB-2 septa are manufactured from high quality, low bleed material using the same exclusive LB-2 rubber formulation that chromatographers are accustomed to using. The difference is that molded septa, unlike traditional die-cut septa, offer easier installation and better sealing inside the injection port. This is because our liqui…
Supelco provides high quality derivatization reagents for silylation.Silylation is the most widely used derivatization technique. Functional groups that present a problem with gas chromatogaphy detection - hydroxyl, carboxylic acid, amine, thiol, phosphate - can be derivatized by silylation reagents. Silylation involves replacement of the acidic hydrogen with an alkylsilyl group, for example, -SiMe3.Silylation derivatives are…
Supelco provides high quality derivatization reagents for acylation.Acylation, an alternative to silylation, allows the conversion of compounds with active hydrogen such as -OH, -SH, and -NH into esters, thioesters and amines, respectively. A very popular example of this method is the insertion of perfluoracyl groups to enable electron capture detection (ECD). Carbonyl groups adjacent to halogenated carbons enhance the ECD res…
