Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, roo…
An innovative, polymeric system for efficient delivery of plasmid DNA and siRNA/miRNA. Experience the transfection breakthrough. Achieve superior transfections with an innovative polymeric system that efficiently delivers both DNA and RNA out of the endosome and into the cytoplasm overcoming a critical barrier to nucleic acid delivery.Head-to-head comparisons against the Lipofectamine® 2000 illustrate superior or equal lucife…
The Thermo Scientific* BRAHMS* PCT Immunoassays are immunofluorescent assays used to determine the concentration of PCT (procalcitonin) in human serum and plasma. The Thermo Scientific* BRAHMS* PCT Immunoassays are intended for use in conjunction with other laboratory findings and clinical assessments to aid in the risk assessment of critically ill patients on their first day of ICU admission for progression to severe sepsis…
The NEBNext® DNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expressi…
he NEBNext® Ultra™ DNA Library Prep Kit for Illumina contains enzymes and buffers that are ideal to convert a small amount of DNA input into indexed libraries for next-generation sequencing on the Illumina platform (Illumina, Inc). The workflow of NEBNext Ultra DNA Library Prep Kit for Illumina is very user-friendly and fast with minimal hands-on time.NEBNext® reagents are a series of highly pure reagents that facilitate samp…
NEBNext® dsDNA Fragmentase® generates dsDNA breaks in a time-dependent manner to yield 100–800 bp DNA fragments depending on reaction time. NEBNext dsDNA Fragmentase contains two enzymes, one randomly generates nicks on dsDNA and the other recognizes the nicked site and cuts the opposite DNA strand across from the nick, producing dsDNA breaks. The resulting DNA fragments contain short overhangs, 5´-phosphates, and 3´-hydroxyl…
The NEBNext® mRNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents…
Next-generation sequencing (NGS) technologies have dramatically reduced sequencing costs and led to novel approaches in many fields of biology.
BioDrop μLITE is the only choice for micro-volume measurement when you need speed without sacrificing accuracy or precision. With no moving parts, the unique in-built sample port offers unrivalled performance delivering a dsDNA detection limit of 1ng/μL. Because the pathlength doesn’t change, the instrument provides excellent measurement reproducibility. Measurements made with the BioDrop μLITE are also highly accurate becaus…
BioDrop DUO UV/Vis spectrophotometer has two measurement modes: a unique in-built sample port unrivalled dsDNA detection limit of 1ng/μl PLUS a cuvette holder for the revolutionary BioDrop CUVETTE 125, ideal for highly concentrated samples. BioDrop DUO is quick and easy to use. It has a large capacitive colour touch screen with on-board software for data acquisition and analysis. Pre-programmed methods for DNA, RNA, oligos and…
A high efficiency, low toxicity, DNA transfection reagent optimized for 293 cellsTransIT®-293 Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in HEK 293 and cell types of associated lineage. TransIT-293 provides all the attributes of the trusted TransIT series of transfection reagents: high transfection efficiency, low toxicity, serum compatibility, simplicity of use…
TransIT-TKO® is a broad spectrum siRNA transfection reagent that enables high efficiency siRNA delivery and knockdown of target gene expression in many cell types including primary cells.
High binding surface is capable of binding medium (>10kD) and large biomolecules that possess ionic groups and/or hydrophobic regions Binding capacity is approximately 400 to 500ng IgG/cm2 Ideal for immunoassays Without lids, lids supplied separately Total volume is 360 µL; recommended working volume of 75 to 200 µL Conforms to standard microplate footprint and dimensions
Round bottom wells with 330 µL total volume Recommended working volumes of 75 to 200 µL Nonreversable lids with condensation rings to reduce contamination Treated for optimal cell attachment Sterilized by gamma radiation and certified nonpyrogenic Individual alphanumeric codes for well identification Individually wrapped
1 ml, one-step RT-PCR kit, includes 2x one-step SYBR Green reaction mix (Taq polymerase, buffers, ROX normalization dyes), iScript reverse transcriptase, and nuclease-free water, for 100 x 20 µl reactions
Incorporating Shimadzu’s proprietary ultrafast technologies (UF Technologies), the new triple quadrupole LCMS-8050 dramatically improves analytical throughput with the ultimate in high-speed performance. In addition, the newly designed ion source and collision cell, Heated-ESI and UFsweeper® III collision cell technology, ensure the highest levels of sensitivity and quantitative performance. Combine with Shimadzu’s wo…
High performance, versatility and ease-of-use in a compact design for efficient mass spectrometry analysis.
The Thermo Scientific™ HyPerforma S.U.B. TK 250L system combines the experience of hundreds of single-use stirred-tank bioreactors used in the industry today with a state-of-the-art controller system for a turnkey bioprocessing solution.This out-of-the-box solution dramatically shortens implementation time and reduces upfront cost while providing all the advantages of our open-architecture single-use bioreactors (S.U.B.). HyP…
The MiSeqDx instrument is the first and only FDA-cleared in vitro diagnostic (IVD) next-generation sequencing (NGS) system. Designed specifically for the clinical laboratory environment, the MiSeqDx instrument offers a small, approximately 4 square feet (0.37 square meters) footprint, an easy-to-follow workflow, and data output tailored to the needs of clinical labs. In addition, the integrated software enables sample trackin…
The ROS-Glo™ H2O2 Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H2O2), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions. This assay allows identification of conditions or test compounds, such as small molecule inhibitors or inducers, that alter ROS levels. The scalable multiwell format couples a stable luminescent si…
Petrifilm™ Rapid Yeast and Mold Count Plates - Rapid, labor-saving technology for product and environmental testing.Yeasts and molds can grow in a wide range of conditions in your raw materials, finished product and production environment, making them difficult to control. Complicating matters is the five-day incubation period required by traditional agar methods. 3M™ Petrifilm™ Rapid Yeast and Mold Count Plates are a faster,…
Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution. Using the conventional trypsin digestion protocol (i.e., overnight incubation at nondenaturing conditions), Trypsin/Lys-C Mix improves protein digestion by eliminating the majority of missed cleavages,…
Polyclonal secondary antibodies raised in goat, rabbit or donkey, immunoaffinity-purified using corresponding immobilized antigens and conjugated to horseradish peroxidase (HRP) enzyme. The Anti-Human IgG (H+L), HRP Conjugate, Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate antibodies bind to both heavy and light chains for all IgG subclasses. As with all antibodies, in certain applications…
Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in addition…
The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification pri…
