DRAQ7
A far-red viability dye staining nuclei in dead / apoptotic cells.
Cell death
A good far-red dye for cell death.
Review Date: 12 Jun 2024 | Biostatus Limited
Analyse cell viability in compound screenings
I use DRAQ5 for cell staining to enable a precise and reliable quantification of the viability of my cells before and after the application of compounds at varying concentrations. It's very easy to use with no need to suspend the stock solution, and readouts of the nuclear stain are always crisp! I have never had any problems with the reproducibility of results, with clear and precise control values every single time. There is honestly nothing bad I can write about this product as I have never had any problems, and it's so simple to use. I do not personally purchase this product, but my team has never had any problems with ordering and delivery, with fast turnaround times.
Review Date: 22 May 2024 | Biostatus Limited
Visualize necrosis on glioblastoma primary spheroids with LSFM imaging
Glioblastoma spheroids of ~1mm in diameter are incubated with Draq7 for 16-20hrs before imaging. Draq7 is visualized in far red and stains dead nuclei, without discriminating cell death pattern. Easily combined with blue and green probes. Photobleaches after 6hrs.
Review Date: 14 Sept 2023 | Biostatus Limited
cell viability assessment
The dye is perfect for detecting dead cells by flow cytometry. Stable, easy to use, quite sensitive.
Review Date: 13 Sept 2023 | Biostatus Limited
Assess nuclei in 2D and 3D immunofluorescence
We use this product to look at nuclei in both 2D and 3D fixed immunofluorescence imaging. Very useful if you do not have access to a 405 laser and cannot use DAPI, as this uses the 647 channel.
Review Date: 4 May 2023 | Biostatus Limited
Analyze ROS on tumor cells
Product is simple and effective. We intend using again.
Review Date: 17 Jun 2022 | Biostatus Limited
Flow cytometry
Easy to use. Reliable results.
Review Date: 3 May 2022 | Biostatus Limited
Nuclear counter stain in deep red
It works well, minimal crosstalk with other fluorescent channels. If you have weak signal use a higher concentration than recommended.
Review Date: 21 Mar 2022 | Biostatus Limited
Flow cell sorting
This product works very well for flow cell sorting.
Review Date: 4 Jan 2022 | Biostatus Limited
Detect double-strand DNA in tissue samples
In our study, cell-impermeable DNA dye DRAQ7 was injected to explore potential host DNA release. This reagent helped us to prove our experiment could induce DNA release easily. We have published our paper!
Review Date: 17 Mar 2021 | Biostatus Limited
DRAQ7 fluoresces in the far-red – with the same spectral properties that have made DRAQ5™ a reagent of choice in HCS.
DRAQ7™ only labels membrane-compromised dead, dying and apoptotic cells and gives clear discrimination between these and healthy cells in high content cytotoxicity and viability assays and in flow cytometry.
The far-red emission, longer wavelength than TOTO-3, permits better combination with other fluorescent probes. It is spectrally compatible with the commonly used orange/red mitochondrial health probes, and UV-excited vital dyes.
DRAQ7 can be used in RNAi knock-down and long-term, dynamic viability assays to report in vitro cytotoxicity and apoptosis.
Also, DRAQ7 efficiently counterstains fixed cell and tissue preparations. It offers the same differential nucl:cyto staining for dual compartment segmentation in translocation tracking and cell morphometrics for in vitro toxicology, that has made DRAQ5™ a favourite of HCS assay developers.
DRAQ7 is supplied as a convenient aqueous, ready-to-use solution. The 1 ml pack size (GBP 140.00 / ca. USD 220.00) is sufficient for at least 200 flow cytometry tests, 300-600 slides and up to 10,000 1536-MTP wells.