Drug discovery > Basic Research Products & Reviews

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System(a,b,c) is designed for this purpose by providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media without prior sample…

The CellTiter-Blue® Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin). Nonviable cells rapidly lose metabolic capacity and thus do not generate a fluorescent signal. The homogeneous assay procedure involves adding the single reagent directly to cells cultur…

The PureYield™ Plasmid Midiprep System is designed to isolate transfection-quality plasmid DNA.   The system provides a rapid method for purification of 100–200μg of plasmid DNA from 50ml bacteria culture. Plasmid DNA can be purified in as little as 30 minutes with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows…

The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. With the Dual-Glo® System, internal controls can be established to minimize s…

Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

The Promega CytoTox 96® Non-Radioactive Cytotoxicity Assay is a colorimetric alternative to radioactive cytotoxicity assays. The CytoTox 96® Assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [51Cr] is released in radioactive assays. Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay that result…

GoTaq® qPCR Master Mix is a new reagent system for use in real-time quantitative PCR (qPCR). The system contains BRYT™ Green, a new proprietary fluorescent DNA binding dye that exhibits greater fluorescence enhancement upon binding to double-stranded DNA than SYBR® Green I. Combined with the GoTaq® Hot Start Polymerase, optimized buffer and proprietary dye, GoTaq® qPCR Master Mix provides robust real-time PCR with increased re…

The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibit…

Oligos from 121 to 180 bases

Disruptive Technology for mAb Screening and Characterization.

Ergonomic upright microscopes with eco-illumination for clinical and laboratory applications.

Single-molecule based super-resolution microscope delivering ten-times the resolution of standard light microscopes.

Newly evolved multiphoton confocal microscopes, which clearly visualize minute structures deep within living organisms.

Igniting New Confocal Imaging Potential - 3D Confocal Imaging with Super Resolution.

Flexible software platform for controlling Nikon microscopes and 3rd-party components, with powerful custom programming tools for image acquisition and analysis.

mRNA sequencing (mRNA-seq) is a potent method for gene expression exploration, it unveils mRNA dynamics, different gene expression and regulatory mechanisms with precision.

The CyFlow® Counter is a fully equipped portable and ultra-compact desktop flow cytometer.

Family of ultra-compact, high-end, multi-laser, benchtop flow cytometry systems.

Your goal: high-quality images that allow measurements and can be accurately repeated with minimal effort. The Leica coded stereo microscopes family helps you to pave the way to correct results.

The next generation STELLARIS confocal platform is your fast track to rewarding research. Power. Potential. Productivity.

With their sophisticated modular design and high-performance fluorescence, the Leica DM2000 microscopes are ideal for complex tasks in pathology, cytology, and many other applications. For special diagnostics requirements, the microscope is certified for in-vitro-diagnostics (IVD) like in-vitro-fertilization (IVF). 

Leica DM2500 & DM2500 LED optical microscopes are tools for demanding tasks in life science routine and research applications. With their transmitted light illumination, optical performance, and state-of-the-art accessories, they are especially well-suited for challenging life science research tasks that require differential interference contrast or high-performance fluorescence.

UC Enuity, the next generation ultramicrotome saves you valuable time and resources through its automated setup functions, providing state-of-the-art sectioning quality.

The Leica DM1000 LED features long-life LED illumination that provides near daylight, bright illumination with constant color temperature and emits less heat. 

The Leica DM750 M is the ideal microscope for basic materials applications in an Industrial Lab or Material Science course. Its versatile stage and reflected light system work in combination to deliver high-quality images of the most demanding specimens.