Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
Millipore now proudly offers the MILLIPLEX® MAP Human Cancer Biomarker Panel. The Cancer Biomarker Panel includes both a Five-Plex assay, which simultaneously measures five serum biomarkers, Macrophage Migration Inhibitory Factor (MIF), Prolactin, CA-125, Leptin and Osteopontin (OPN), and a Single Plex IGF-II assay. The combination of the six serum biomarkers that discriminate between disease-free and ovarian cancer patien…
Aitken Scientific have created a software product called A-S Login (fully validated to GAMP standards) which will enable the pharmaceutical industry and its suppliers to minimise cost and time when implementing the requirements of 21 CFR Part 11. • Maintains permitted applications, allowing versions and access rights to be controlled. • Maintains the list of authorised users o Password administration…
A simple 7-minute sample preparation procedure is the first part of a complete workflow provided by the TaqMan® Gene Expression Cells-to-CT™ Kit. Remove the guesswork of assembling separate RNA purification, RT, and real-time PCR kits, and achieve unsurpassed performance and sensitivity in gene expression analysis, including detection of siRNA-induced mRNA knockdown. - Complete solution—Optimized workflow includes cell lysis r…
ACTOR™ (Automated crystal transport orientation and retrieval robot) is a proven commercial solution for automating routine crystal screening and data collection at both synchrotron beam lines and home laboratories. Compatible with almost any goniometer and detector combination, the ACTOR eliminates much of the physical handling of samples required during crystal screening and data collection. ACTOR allows you to screen up to…
Reagent kit that enables rapid lysis of yeast cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases Application Use Complete Lysis-Y to rapidly lyse cells of various yeast strains (e.g., Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris) in only 20 minutes at room temperature and simultaneously inhibit protease activity in the cell ly…
Reagent kit that enables rapid lysis of bacteria for highly efficient protein extraction and concurrent protection of extracted proteins against a multitude of proteases. Suitable for downstream purification using IMAC. Application Use Complete Lysis-B (2x), EDTA-free to rapidly lyse bacteria in only 10 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein extraction…
Reagent kit that enables rapid lysis of bacteria for highly efficient protein extraction and concurrent protection of extracted proteins against a multitude of proteases Application Use Complete Lysis-B (2x) to rapidly lyse bacteria in only 10 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein extraction and concurrent inhibition of a multitude of proteases, includ…
Reagent kit that enables rapid lysis of mammalian cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases. Suitable for downstream purification using IMAC. Application Use Complete Lysis-M, EDTA-free to rapidly lyse mammalian cells in only 5 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein e…
Reagent kit that enables rapid lysis of mammalian cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases Application Use Complete Lysis-M to rapidly lyse mammalian cells in only 5 minutes and simultaneously inhibit protease activity in the cell lysate. The kit enables highly efficient protein extraction and concurrent inhibition of a multitude of proteas…
Reagent kit that enables rapid lysis of yeast cells for highly efficient protein extraction and simultaneous protection of extracted proteins against a multitude of proteases. Suitable for downstream purification using IMAC. Application Use Complete Lysis-Y, EDTA-free for the rapid cell lysis of various yeast strains (e.g., Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris) in only 20 minutes at room tempera…
Fluorescein-conjugated anticoagulant for the detection of phosphatidylserine in the outer leaflet of apoptotic cellsProduct Description Detection: Annexin-V-FLUOS can be directly detected in FACS analysis and immunochemistry without a secondary detection system. Sample material: Cell lines and freshly isolated cells. Specificity: Annexin-V binds in a calcium-dependent manner to negatively charged phospholipid surfaces, and sho…
Biotin-conjugated anticoagulant for the detection of phosphatidylserine in the outer leaflet of apoptotic cellsProduct Description Detection: Annexin-V-Biotin can be used in immunohistochemistry with a secondary detection system (e.g., Streptavidin-POD conjugate). Sample material: Cell lines and freshly isolated cells Specificity: Annexin-V binds in a calcium-dependent manner to negatively charged phospholipid surfaces, and…
Alexa 568-conjugated anticoagulant for the detection of phosphatidylserine on the outer leaflet of apoptotic cellsBenefits Detection flexibility: Analysis of results with flow cytometry and/or fluorescence microscopy. Alexa is a red dye, which can be used for double or triple staining with other fluorescent markers (e.g., fluorescein-labeled surface markers). Accuracy: Accurately distinguish apoptotic cells from necrotic…
Kit for the detection and quantification of apoptosis and differentiation from necrosis at the single-cell level, based on Annexin-V-labelingProduct Description Detection: Annexin-V-FLUOS can be directly detected in FACS analysis and immunochemistry without a secondary detection system. Sample material: Cell lines and freshly isolated cells. Specificity: Annexin-V-FLUOS binds in a Ca2+-dependent manner to negatively charged ph…
Roche Cell Death Detection ELISA - Photometric enzyme immunoassay for the quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) after induced cell deathBenefits of Roche Cell Death ELISA: High sensitivity: (5 x 102 cells/ml) Fast performance: (5 - 6 hours) No prelabeling of cells necessary Nonradioactive assay system No species restrictions Easy handling…
Kit for the detection and quantification of apoptotic cell death on a single-cell level by light microscopy in immunocyto- and immunohistochemistry Benefits Sensitive: The maximum intensity of labeling (cell staining) of apoptotic cells is higher than the nick translation method. Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction, but before the addition of the secondary d…
Kit for the detection and quantification of apoptotic cell death on a single-cell level by flow cytometry and fluorescence microscopy, and for double labeling with fluorescein-labeled cell markers (TMR red)Benefits Convenient: No secondary detection system required. Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis. Flexible:…
Kit for the detection and quantification of apoptosis (programmed cell death) at the single-cell level, based on labeling of DNA strand breaks (TUNEL technology): Analysis by fluorescence microscopy or flow cytometry Benefits Sensitive: The direct labeling procedure using fluorescein-dUTP reduces background labeling. Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction. Co…
Application The Cellular DNA Fragmentation ELISA may be applied to measure: Apoptosis by detection of BrdU-labeled DNA fragments in the cytoplasm of affected cells (= nonradioactive alternative to the [3H]-thymidine-based DNA-fragmentation assay). Cell-mediated cytotoxicity by detection of BrdU-labeled DNA fragments released from damaged target cells into the culture supernatant (= nonradioactive alternative to the [3H]-thymid…
Application The kit is used for the detection of BrdU incorporated into cellular DNA by immunocyto/histochemistry. Benefits Safe: No radioisotopes are used. Easy to perform: Follows a standard immunohistochemistry protocol. Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU. Flexible: Allows double-labeling protocols.
Application The kit is used for the detection of BrdU incorporated into cellular DNA using immunofluorescence microscopy. It is used for the detection of DNA synthesis by either in vitro labeling of cells or organ cultures, or by in vivo labeling, in which frozen or paraffin-embedded tissue sections must be prepared prior to fixation. Benefits Safe: No radioisotopes are used. Easy to perform: Follows a standard immunof…
Application The Cell Proliferation ELISA, BrdU (colorimetric) belongs to the second, improved generation of kits for measuring DNA synthesis. It is designed as a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell syst…
ApplicationThe In Situ Cell Proliferation Kit, FLUOS belongs to the second generation of improved kits for the detection of DNA synthesis at the single-cell level (in situ) by immunocyto-/immunohistochemistry. The kit can be used in many different in vitro and in vivo cell systems, for example: Detection and quantification of cells in S-phase Determination of growth fractions for tumors in animal-model systems Detectio…
ApplicationThe immunoreactivity of the M30 antibody is confined to the cytoplasm of apoptotic cells. It can be easily used for: Immunocytochemistry Flow Cytometry Immunohistochemistry: paraffin-embedded tissue sections (secondary enhancer reagents needed for this particular application) and cryostat sections Benefits Determine caspase activity, even in formalin-embedded tissue. Identify the stage of apoptosis (…
ApplicationSamples suitable for the Caspase 3 Activity Assay are: Lysates of apoptosis-induced cells Recombinant caspase 3 (CPP32) The Caspase 3 Activity Assay can be used to: Detect and quantify in vitro caspase 3 activity in cell lysates Analyze caspase 1, 2, 3, or 7 activity by simply omitting the capture-antibody step Perform inhibitor studies for Caspase 3 This kit is intended as a tool for life science…
