Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
Application The kit is used for the detection of BrdU incorporated into cellular DNA using immunofluorescence microscopy. It is used for the detection of DNA synthesis by either in vitro labeling of cells or organ cultures, or by in vivo labeling, in which frozen or paraffin-embedded tissue sections must be prepared prior to fixation. Benefits Safe: No radioisotopes are used. Easy to perform: Follows a standard immunof…
Application The Cell Proliferation ELISA, BrdU (colorimetric) belongs to the second, improved generation of kits for measuring DNA synthesis. It is designed as a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell syst…
ApplicationThe In Situ Cell Proliferation Kit, FLUOS belongs to the second generation of improved kits for the detection of DNA synthesis at the single-cell level (in situ) by immunocyto-/immunohistochemistry. The kit can be used in many different in vitro and in vivo cell systems, for example: Detection and quantification of cells in S-phase Determination of growth fractions for tumors in animal-model systems Detectio…
ApplicationThe immunoreactivity of the M30 antibody is confined to the cytoplasm of apoptotic cells. It can be easily used for: Immunocytochemistry Flow Cytometry Immunohistochemistry: paraffin-embedded tissue sections (secondary enhancer reagents needed for this particular application) and cryostat sections Benefits Determine caspase activity, even in formalin-embedded tissue. Identify the stage of apoptosis (…
ApplicationSamples suitable for the Caspase 3 Activity Assay are: Lysates of apoptosis-induced cells Recombinant caspase 3 (CPP32) The Caspase 3 Activity Assay can be used to: Detect and quantify in vitro caspase 3 activity in cell lysates Analyze caspase 1, 2, 3, or 7 activity by simply omitting the capture-antibody step Perform inhibitor studies for Caspase 3 This kit is intended as a tool for life science…
ApplicationDuring apoptosis, the activation of specific proteases, called Caspases (cysteinyl-aspartic-acid-proteases), is one of the initial intracellular biochemical events. The Homogeneous Caspases Assay can be used to detect caspase activity in life science research applications: for sensitive quantification of activated caspases for screening of caspase inhibitors as an automatable one-step assay for high-throughp…
X-tremeGENE siRNA Transfection Reagent efficiently delivers short interfering RNA (siRNA) into many commonly used cell types including HeLa, NIH 3T3, HEK-293, CHO-K1, and COS-7, and several hard-to-transfect cell lines such as HT29, a human adenocarcinoma cell line. When used for cotransfection-based gene knockdown, X-tremeGENE siRNA Transfection Reagent enables significant protein expression and effective knockdown levels due…
Use G-418 antibiotic to select and maintain eukaryotic cells that are stably transfected with the genes for neomycin resistance. High purity: Use less antibiotic and produce greater selection pressure while minimizing toxicity from contaminants. Convenient sterile-filtered liquid format: Reduce time, effort, and potential errors by using a prepared sterile-filtered solution instead of making stock solutions from powder.…
Upgrade from the Rapid DNA Ligation Kit and choose the new Rapid DNA Dephos & Ligation Kit for fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments. The convenient kit enables ligation of DNA in 5 minutes and dephosphorylation in 10 minutes. This kit contains the new recombinant rAPid Alkaline Phosphatase, the tool of choice for dephosphorylation of 5' phosphates from nucleic acids. This kit…
The Transcriptor First Strand cDNA Synthesis Kit is designed to reverse transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources for the following applications: Studying gene expression levels, via two-step RT-PCR, using qualitative RT-PCR or quantitative RT-PCR on the LightCycler® Carousel-Based System, the LightCycler® 480 System, or other real-time instruments. Generating cDN…
The Transcriptor High Fidelity cDNA Synthesis Kit is designed to reverse transcribe RNA with an increased fidelity compared to other reverse transcriptases. The kit features Transcriptor High Fidelity Reverse Transcriptase, a blend of a recombinant reverse transcriptase and a proofreading mediating enzyme optimized for two-step RT-PCR, making this the product of choice for the following applications: Cloning genes of inter…
The NA-Star® Influenza Neuraminidase Inhibitor Resistance Detection Kit includes everything you need to quantitate neuraminidase activity and neuraminidase inhibitor resistance in avian, equine, human (types A and B), and porcine influenza viruses. The kit's fast and easy protocol and convenient 96-well plate format make it ideal for monitoring influenza virus neuraminidase inhibitor resistance, as well as high-throughput inhi…
Dual-Light® luminescent reporter gene assay is designed for the rapid and sensitive detection of firefly luciferase and b-galactosidase in a single extract aliquot for gene expression assays. Detection of firefly luciferase and b-galactosidase in the same sample simplifies normalization of transfection efficiency Wide dynamic range of b-galactosidase and luciferase assays enables accurate measurement of both enzymes from…
The Galacto-Light™ beta-Galactosidase Reporter Gene Assay System is designed for the rapid, ultrasensitive detection of b-galactosidase in reporter gene assays.
The Galacto-Light Plus™ beta-Galactosidase Reporter Gene Assay System is designed for the rapid, ultrasensitive detection of b-galactosidase in reporter gene assays.
The Galacto-Star™ One-Step beta-Galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of B-galactosidase in cell lysates.
The Gal-Screen® assay system combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.
The Luc-Screen® assay system with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme.
IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage. IPTG is also used to induce the expression of cloned genes that are under control of the lac operon.
X-GAL (5-Bromo-4-Chloro-3 Indolyl-Beta-D-thiogalactopyranoside) is a chromogenic substrate for Beta-galactosidase that turns blue upon hydrolysis and is regularly used for the blue/white color selection assay. Bacteria with a lac+ phenotype grow as blue colonies on plates containing X-GAL.
The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression.
Recombinant Human IFN Alpha Sampler, 12 individual subtypes including: Human IFN-alpha A, B2, C, D, F, G, H2, I, J1, K, 4b, WA Catalog Number: 11001-1 Activity: Request Info Source: E. Coli Accession Number: Request info Size: 1 kit Purity: ≥ 95% Formulation: Frozen with a carrier protein Protein Synonyms: IFN, Interferon, Type I Interferon, Sampler, SubtypeShipping Conditions: Dry ice Storage Temp.: -20˚C/-70°C Pre…
Recombinant Human IFN Alpha A (Alpha 2a), Gene IFNA2 Catalog Number: 11100-1 Activity: 8.7 x 108 u/mgSource: E. Coli Accession Number: V00549Size: 5 x 106 unitsPurity: ≥ 95% Formulation: Frozen with a carrier protein Protein Synonyms: IFN, Interferon, Type I Interferon, Subtype Shipping Conditions: Dry ice Storage Temp.: -20˚C/-70°C Preservative: N/A Shelf Life: 2 years from date of receipt 2007 Retail price (Effectiv…
Recombinant Human IFN Alpha A (Alpha 2a), Gene IFNA2 Catalog Number: 11101-2 Activity: 8.7 x 108 u/mg Source: E. Coli Accession Number: V00549 Size: 20 µg Purity: ≥ 95% Formulation: Frozen without a carrier protein Protein Synonyms: IFN, Interferon, Type I Interferon,Subtype Shipping Conditions: Dry ice Storage Temp.: -20˚C/-70°CPreservative: N/A Shelf Life: 2 years from date of receipt 2007 Retail price (Effective 10/1/2007):…
