Flow Cytometry / Cell Counting Products & Reviews

Hoechst 33342 Ready Flow Reagent is a bright, easy-to-use cell-permeable stain for cell cycle analysis on a flow cytometer. When bound to double-stranded DNA, the dye emits blue fluorescence when excited with UV light, with an emission maximum at 461 nm.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteCell-permeable and effective in fixed and live-cell staining for cell cycl…

FxCycle Violet Ready Flow Reagent is a bright, ready-to-use dsDNA stain for cell cycle analysis in fixed cells using flow cytometry. The cell-impermeable dye binds ds DNA after cells are fixed, eliminating RNase treatment. It is excitable by both UV and 405 nm lasers with an emission peak at 461 nm. FxCycle Violet Ready Flow Reagent is an ideal choice for DNA content analysis and can be included in multi-color cell-cycle studi…

The AbC Anti-Mouse Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse antibodies with any laser in flow cytometry. It addresses issues associated with cellular-based compensation controls such as poorly expressed antigens and dimly stained cells.FeaturesSpecies reactivity—for use with all isotypes of…

The AbC Total Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse, rat, hamster, or rabbit antibodies with any laser in flow cytometry. It addresses issues associated with cellular-based compensation controls such as poorly expressed antigens and dimly stained cells.FeaturesBroadest multispecies react…

ArC Amine Reactive Compensation Bead Kit is a ready-to-use, bead-based compensation tool specifically optimized for use with LIVE/DEAD™ Fixable dead cell stain kits. The kit contains two components: the ArC reactive beads (Component A) bind any of the amine-reactive dyes and provide a positive signal; the ArC negative beads (Component B) have no reactivity and provide a negative compensation control. Results are analyzed by fl…

CountBright™ Absolute Counting Beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range from UV to 635 nm excitation wavelengths and 385-800 nm emission wavelengths. CountBright™ Absolute Counting Beads can be used to compare bead events to cell events using flow cytometry, allowing the absolute number of cells in a sample to be calculated. Cell counts are more accurate and less compl…

The Flow Cytometry Size Calibration Kit uses nonfluorescent particle-size calibration standards for a simple, accurate way to determine cell sizes by flow cytometry. The kit contains six suspensions of highly uniform polystyrene microspheres with different diameters: 1.0 µm, 2.0 µm, 4.0 µm, 6.0 µm, 10.0 µm and 15.0 µm. All of the microsphere suspensions are provided in convenient dropper vials.

The LIVE/DEAD BacLight Bacterial Viability and Counting Kit allows researchers to reliably distinguish and quantitate live and dead bacteria with the aid of a flow cytometer, even in a mixed population containing a range of bacterial types. This kit uses a mixture of two nucleic acid stains - green-fluorescent SYTO™ 9 dye and red-fluorescent propidium iodide - for viability determinations, and a calibrated suspension of micros…

The LIVE/DEAD FungaLight Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO™ 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. SYTO 9 stain generally labels all yeast in a population, with or without intact membranes. Propidium iodide penetrates only…

The pHrodo™ Green E. coli BioParticles™ Phagocytosis Kit for Flow Cytometry enables the detection of phagocytic activity in whole blood samples and live cell lines using flow cytometry. With no need for quenching and wash steps, the kit saves time and minimizes uncertainty over particle signals from internalized particles.The pHrodo™ Green E. coli BioParticles™ assay provides sensitive detection without the need for quenching…

The pHrodo Green S. aureus BioParticles™ Phagocytosis Kit for Flow Cytometry enables the detection of phagocytic activity in whole blood samples and live cell lines using flow cytometry. With no need for quenching and wash steps, the kit saves time and minimizes uncertainty over particle signals from internalized particles.The pHrodo Green S. aureus BioParticles assay provides sensitive detection without the need for quenching…

The LIVE/DEAD Fixable Blue Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Blue Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells wit…

The LIVE/DEAD Fixable Violet Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Violet Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells…

The LIVE/DEAD Fixable Aqua Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Aqua Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells wit…

The LIVE/DEAD Fixable Yellow Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Yellow Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells…

The LIVE/DEAD Fixable Green Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Green Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells w…

The LIVE/DEAD Fixable Red Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Red Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cells with…

The LIVE/DEAD Fixable Far Red Dead Cell Stain Kit provides the stable components to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. The amine-reactive LIVE/DEAD Fixable Far Red Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines in cel…

Propidium Iodide Ready Flow™ Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. Propidium iodide can only enter cells that have lost membrane intergrity (e.g., dead cells). Once inside, it can bind with nucleic acids, emtting a red fluorescence, which has excitation/emission maxima of 535/617 nm. Propidium iodide is commonly excited with the 488 nm laser,…

TO-PRO3 Ready Flow Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. TO-PRO3 Ready Flow Reagent can only enter cells that have lost membrane intergrity (e.g., dead cells). Once inside, it can bind with nucleic acids, emitting a red fluorescence, which has an excitation/emission maxima of 642/661 nm, similar to Alexa Fluor™ 647 or allophycocyanin (APC).Fe…

SYTOX AADvanced Ready Flow Reagent is a high-affinity nucleic acid stain for the detection of dead cells in flow cytometry. SYTOX AADvanced Ready Flow Reagent can only enter cells that have lost membrane intergrity (e.g., dead cells). Once inside, it can bind with nucleic acids, emitting a red fluorescence, which has an excitation/emission maxima of 546/647 nm. The SYTOX AADvanced Ready Flow Reagent dye is spectrally similar t…

SYTOX™ Dead Cell Stain Sampler Kit contains sample sizes of five nucleic acid stains to identify the most appropriate satin to work in multi-color experiments. Stains include: SYTOX Blue (for the 405 nm violet laser), SYTOX Green (for the 488 nm blue laser with green emission), SYTOX Orange (for the 532 nm green laser), SYTOX AADvanced (for the 488 nm blue laser with red emission), and SYTOX Red (for the 633/5 nm red laser).St…

Annexin V Alexa Fluor 488 Ready Flow Conjugate is a bright, easy-to-use stain for apoptosis with high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis. The Annexin V Alex Fluor 488 conjugate is excitable at 495 nm with an emission peak at 519 nm.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteCan be added directl…

Annexin V Alexa Fluor 647 Ready Flow Conjugate is a bright, easy-to-use stain for apoptosis with high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis. The Annexin V Alex Fluor 647 conjugate is excitable at 650 nm with an emission peak at 665 nm.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteCan be added directl…

Annexin V APC Ready Flow Conjugate is a bright, easy-to-use stain for apoptosis with high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis. The Annexin V APC conjugate is excitable at 650 nm with an emission peak at 660 nm.FeaturesStable at room temperatureConvenient, ready-to-use format—no need to dilute, weigh, or pipetteCan be added directly to Ca2+-containing me…