Enzyme Activity Assays
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-9 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the LEHD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate LEHD-pNA. The free pNA can be quantified using a spectropho…
The CHEMICON Proteasome Activity Assay Kit provides a quick, efficient and sensitive system for evaluation of proteasome activity in cell lysates or inhibitor screening. Chemicon's Proteasome Activity Assay Kit provides a simple and convenient means for assaying proteasome activity that recognize the substrate LLVY (Meng et al. 1999). The assay is based on detection of the fluorophore 7-Amino-4-methylcoumarin (AMC) after cle…
The CHEMICON uPA Activity Assay Kit provides a quick, efficient and sensitive system for evaluation of uPA activity and for screening of uPA inhibitors. The assay is colorimetric and does not require radioactivity or fluorescence equipment. The assay is sensitive over a range of 0.05-50 units of uPA activity. The CHEMICON uPA Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA. Addition of this…
Innova Biosciences, based on the Babraham Research Campus, Cambridge UK, has launched a family of assay reagents called PiColorLock™, to facilitate the measurement of the activity of phosphatases, ATPases and other Pi-generating enzymes in microplates. Unlike the traditional "Malachite Green" method, which is prone to precipitation problems, PiColorLock reagent is specially formulated to give a stable end-point signal, meani…
ColorKits are designed to facilitate the measurement of the activity of phosphatases, ATPases and other Pi-generating enzynes in microplate assays. Each Kit contains a phosphorylated substrate for a particular class of enzyme, together with a carefully optimised PiColorLock detection reagent, Pi standard, 2x96-well plates and a selection of buffer concentrates for optimisation of assay conditions with the enzyme of interest.…
PiColorLock Drug Discovery Kits are designed to facilitate the measurement of the activity of potential inhibitors of phosphatases, ATPases and other Pi-generating enzymes in microplates. The Kits combine a purified recombinant human enzyme target, phosphorylated substrate, Pi standard and 2 multiwell plates. Each Kit is also supplied with a carefully optimised PiColorLock detection reagent and a selection of buffer concentr…
The SIRTainty™ Class III HDAC Assay utilizes a novel patent pending technology for the sensitive detection of all known sirtuin family members. This easy to perform assay employs nicotinamidase to measure nicotinamide generated upon cleavage of NAD+ during sirtuin mediated deacetylation of a substrate providing a direct assessment of the activity of class III HDAC enzymes. Unlike conventional assays that are dependent upon a s…
Simple, sensitive method for detecting changes in histone methylation levels from purified core histones or histones isolated by acid extraction
lyophilized powder, 10 units/mg solid
For screening of hyaluronidase inhibitors, and quantification of hyaluronidase activity in cells and other biological fluids.
Combining the sensitivity of a fluorescence-based assay with a microplate format enables a rapid, quantitative readout suitable for high-throughput analysis. In a microplate well, the fluorescent signal can be generated within whole cells, in cell lysates, or in purified enzyme preparations and may then be analyzed by measuring fluorescence intensity from the well without the need for cellular imaging.
Meet the Gallery Plus Enzyme Master - the first automated enzyme analyzer designed specifically for enzyme assay applications, with flexibility and automation Thanks to custom designed software, all reagent additions, incubations, and measurements are fully-automated, which saves time, eliminates manual error, and improves method robustness. And with reliable instrumentation, you can facilitate a simple method transfer from R…
Meet the Gallery Enzyme Master - the first automated enzyme analyzer designed specifically for enzyme assay applications. Combining robust hardware and new custom designed software, this best-in-class solution delivers fully-automated incubation settings, reagent additions, and precise measurement calculations—all with a touch of a button. This enzyme analyzer offers the smartest (and fastest) way yet to streamline method dev…
An acetyl-biotinylated substrate recognized by a Eu3+ anti-acetyl MAb and SA-XL665, which is used in activator screening or to test an High Throughput SIRT1 inhibitor. The level of enzymatic activity is inversely proportional to the amount of signal produced in the system. For more information please visit Cisbio Bioassays website.
