HTS Assay Kits Products & Reviews

Three kits to cover a wide range of cAMP concentrations.After the introduction of IP-One, the first HTS immunoassay that can easily detect inositol(1)phosphate (IP1), a stable second messenger which tightly correlates with Gq-coupled receptor activity, the optimization of cAMP assays with the new HTRF® acceptor d2 offers a complete GPCR platform.The upgraded cAMP dynamic 2 and cAMP femto 2 assays, along with the new cAMP HiRa…

Application The Cytotoxicity Detection KitPLUS (LDH) is a fast, sensitive, and simple method to quantitate cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells using the 96-well or 384-well plate format. The kit can be used in many different in vitro cell systems when damage to the plasma membrane occurs. For example: Detection and quantification of cell-mediated cytotoxicity. Deter…

BellBrook Labs Transcreener ® UDP² Glycosyltransferase Assays

Cyto-Cellect™ Human IgGK Detection Kit offers a sensitive and robust assay for the detection of human IgG antibody secretion, ideal for streamlining cell isolation and titer determination.

Outstanding GMP QC Testing Services offering Bioassays throughout the lifecycle of drug development, to support the release, stability, and in-process testing of your drug.

Ready-to-use, far-red fixed cell counterstain

The ONE-Glo™ + Tox Assay combines luciferase assay chemistry with a cell viability marker to better understand reporter gene expression in the context of cell health. The assay uses a two-step, addition-only process to make these measurements in a single well of a plate, negating the need to run parallel assays. The first part of the assay is a nonlytic fluorescence assay (CellTiter-Fluor™ Cell Viability Assay) that measure…

LDH-Glo™ Cytotoxicity Assay detects cell death from a small number of cells, including 3D cell cultures, based on the release of lactate dehydrogenase.

The RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay provides a no-wash, one-step Annexin V assay that allows continual monitoring of cell state to determine apoptotic onset using a plate-reading luminometer.

The GSH/GSSG-Glo™ Assay is a bioluminescent assay to detect and quantitate total glutathione ratios.

IRDye 680RD is the near-infrared fluorescent dye of choice for applications in the 700 nm wavelength, especially Western blots, cell-based assays such as the In-Cell Western assay, and small animal imaging experiments.

IRDye Infrared Dyes and near-infrared (NIR) fluorescent imaging deliver enhanced sensitivity due to low background autofluorescence in the near-infrared region, therefore, giving a higher signal to noise ratios.

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes.

NanoLuc® and NanoBiT® Luciferase Substrate for Animal Imaging Studies​

The Lucica method for Glycated Albumin (GA), manufactured by Asahi Kasei Pharma Corporation, is a specific test for GA that is now FDA cleared for sale in the U.S. The Lucica test is distributed exclusively by EKF USA (former Stanbio). This test is one of the most widely used and published GA methods worldwide. 

Gyrolab Bioaffy 4000 CD, the newest member of the Gyrolab Bioaffy CD family, offers 2-6 fold extended sensitivity for PK and biomarker analysis compared to Gyrolab Bioaffy 1000.

The Mosaic ELISA Human Cytokine Panel 1 is an excellent tool for the detection of 8 different cytokines in the same sample. Mosaic ELISA kits employ multiplex microarray technology to provide an accurate, efficient, and economical alternative to conducting multiple traditional ELISA experiments. Cytokines are pleiotropic extracellular signaling molecules that influence myriad physiological and pathological processes. Released…

EMD Millipore's hERG membrane preparations are crude membrane preparations made from HEK293 stable recombinant cell lines, which are ideal HTS tools for screening antagonists against the hERG channel. The membrane preparations exhibit a Kd of 5.2 nM for [3H]-Astemizole. With 10 μg/well hERG Membrane Prep and 3.0 nM [3H]-Astemizole, a greater than 4-fold signal-to-background ratio was obtained.

EMD Millipore's cAMP HTS immunoassay kit, 384 well is a competitive immunoassay for quantitative chemiluminescence-based detection of cAMP in cell lysates and supernatants, which has been optimized for use in high throughput screening applications. The kit provides two ready to use 384 well pre-coated assay microplates and all reagents required to perform the assay.

The passive reference dye can be added to fluorescence-based quantitative PCR (qPCR) assays to compensate for non-PCR related variations in fluorescence. Product comes in 10 vials of 100 µL. 1mM for controlling the final dye concentration.

The ProteoPlex™ 16-Well Human Cytokine Array Kit is designed for multiplex detection and measurement of 12 important human cytokines in parallel from up to 15 experimental samples. The combination of advanced surface chemistry, well-characterized capture agents, and a highly-sensitive fluorescence-based detection system provides a robust method for comparing relative cytokine abundance or cytokine quantification in serum sampl…

An easy way to assess tag accessibilityThe production of fusion proteins often raises the issues not only of their structural integrity after isolation, but also of the accessibility of the associated tag. In the case of GST or 6HIS tagged proteins, this verification can be achieved with HTRF® GST and 6HIS check kits. These two assays are competitive immunoassays in which the anti-GST or anti-6HIS Cryptate conjugates bind dire…

A rapid and sensitive homogeneous assay for cortisol assessmentThe HTRF® cortisol assay can be used for the quantification of cortisol in rodent serum, or directly on cells. This assay has also been validated for 11-beta HSD1 screening on whole cells or microsomes.The assay requires only 2 reagent additions, followed by an incubation period of 2 hours at room temperature. It has a detection limit of 70 pg/mL (e.g. 2 fmol/well…

A straightforward tool for biomolecular labeling with Europium cryptateThe Cryptate labeling kit includes all the necessary materials to allow rapid Cryptate labeling of various biomolecules and purification. The labeling reagent Eu3+-TBP-NHS Cryptate is suitable for labeling peptides, proteins, and oligonucleotides, as well as small molecules containing amino groups (N-terminus or lysine residues). The labeling procedure requ…