Kinase Glo® Assay
Promega Corp.Universal, HTS Kinase Screening Assays
Use higher ATP concentrations: Linear response up to 500μM ATP.
Use any kinase and kinase-substrate combination, including peptide, protein, lipid, and sugar substrates.
Perform the assay without substrate modifications.
Obtain reliable results: Z´-factor values routinely >0.7.
Reduce false hits: Luminescence is much less susceptible to interference from library compounds t…