Cell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.
The Corning 3D Clear Washing Buffer 10X is used for the Corning 3D Clear immunolabeling process. This solution is a concentrate and is comprised of: PPBS with 0.2% Tween-20 and 10µg/mL heparin. This solution should be diluted to 1X concentration with PBS prior to use.Rapid tissue clearingEasy to UseNo Special Equipment RequiredCompatible with IF, FP and other Fluorescent lablesMicroplate and Automation CompatibleReversible to…
The Corning 3D Clear Washing Buffer 10X is used for the Corning 3D Clear immunolabeling process. This solution is a concentrate and is comprised of: PPBS with 0.2% Tween-20 and 10µg/mL heparin. This solution should be diluted to 1X concentration with PBS prior to use.Rapid tissue clearingEasy to UseNo Special Equipment RequiredCompatible with IF, FP and other Fluorescent lablesMicroplate and Automation CompatibleReversible to…
The Agilent Seahorse XF T Cell Metabolic Profiling kit allows for robust and accurate measurements of both glycolytic and mitochondrial activities in T cell populations, providing a complete picture of T cell energy metabolism. These measurements can be linked to antitumor properties of T cell therapy products and are therefore valuable in designing and optimizing therapy development processes to improve T cell persistence or…
Agilent Seahorse Analytics is the newest data analysis application for Agilent Seahorse XF Analyzer products. Seahorse Analytics is built in the cloud, meaning there is no software for you to install, update, or manage. Learn how Seahorse Analytics can make your data analysis experience easier and more enjoyable. Register for a free account and start uploading your assay result files now.
The Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP) is a proprietary reagent that permeabilizes intact cells in culture. It selectively targets the cellular plasma membrane while leaving the mitochondrial membrane intact. This selectivity enables the detailed characterization of key components in mitochondrial function including transporters, enzymes, and electron transport chain complexes.
The Agilent Seahorse XF Palmitate Oxidation Stress Test Kit provides validated reagents to measure long chain fatty acid oxidation (FAO) via assessing changes in oxygen consumption (OCR) in intact cells. The kit includes the XF Palmitate-BSA FAO Substrate, L-carnitine, etomoxir, as well as oligomycin, FCCP, and rotenone/antimycin A to determine the intrinsic rate and capacity of a cell to oxidize palmitate in the absence or l…
Selvita’s in vivo team has long standing experience in the areas of infection, inflammation and fibrosis and has contributed to numerous preclinical and clinical candidates. Over 60 animal models in mice, rats, and rabbits are fully characterized and validated with clinically relevant pharmacological controls whereas in-depth expertise in translational biomarker selection and validation gives confidence in the smooth transi…
TruCytes™ TBNK synthetic cells are lyophilized cell mimics that feature T cell, B cell and NK cell markers with scatter coordinates (FSC and SSC) that closely mimic lymphocyte, monocyte, and granulocyte populations. This product is intended to provide positive and negative signal detection for specific surface biomarkers targeted by specific antibodies. Biomarkers: CD4+ T-cells: CD45+, CD3+, CD4+ CD8+ T-cells: CD45…
Gentronix has launched a new, accurate and fast in vitro mammalian cell genotoxicity assay - GreenScreen HC. The TK6 host cells are p53 competent and familiar to most genetic toxicology laboratories. A patented GFP reporter system exploits the proper regulation of the GADD45a gene. The assay delivers both high specificity and high sensitivity and detects all common mechanistic classes of genotoxin. The 96-well microplate for…
IP-One ELISA assay kit from Cisbio Bioassays - Simple and highly accurate measurement of IP1 IP1, a downstream metabolite of IP3, accumulates in cells following activation of Phospholipase C (PLC) coupled receptors. Among them, the Gq coupled GPCRs represent the most important family of receptors which can activate the β subtype of the PLC family. Other receptor types, like protein tyrosine kinase receptors, antigen or immuno…
Millipore's full-length mTOR kinase is now available as part of the KinaseProfiler™ Service. This kinase is the first biologically relevant mTOR to be commercially available. The KinaseProfiler Service offers 264 kinases for selectivity profiling, using a catalytic radiometric assay format. The addition of the full-length mTOR to the KinaseProfiler panel provides high-quality, confidential screening of compounds against this c…
Fluorescein-conjugated anticoagulant for the detection of phosphatidylserine in the outer leaflet of apoptotic cellsProduct Description Detection: Annexin-V-FLUOS can be directly detected in FACS analysis and immunochemistry without a secondary detection system. Sample material: Cell lines and freshly isolated cells. Specificity: Annexin-V binds in a calcium-dependent manner to negatively charged phospholipid surfaces, and sho…
Biotin-conjugated anticoagulant for the detection of phosphatidylserine in the outer leaflet of apoptotic cellsProduct Description Detection: Annexin-V-Biotin can be used in immunohistochemistry with a secondary detection system (e.g., Streptavidin-POD conjugate). Sample material: Cell lines and freshly isolated cells Specificity: Annexin-V binds in a calcium-dependent manner to negatively charged phospholipid surfaces, and…
Alexa 568-conjugated anticoagulant for the detection of phosphatidylserine on the outer leaflet of apoptotic cellsBenefits Detection flexibility: Analysis of results with flow cytometry and/or fluorescence microscopy. Alexa is a red dye, which can be used for double or triple staining with other fluorescent markers (e.g., fluorescein-labeled surface markers). Accuracy: Accurately distinguish apoptotic cells from necrotic…
Roche Cell Death Detection ELISA - Photometric enzyme immunoassay for the quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) after induced cell deathBenefits of Roche Cell Death ELISA: High sensitivity: (5 x 102 cells/ml) Fast performance: (5 - 6 hours) No prelabeling of cells necessary Nonradioactive assay system No species restrictions Easy handling…
Kit for the detection and quantification of apoptotic cell death on a single-cell level by light microscopy in immunocyto- and immunohistochemistry Benefits Sensitive: The maximum intensity of labeling (cell staining) of apoptotic cells is higher than the nick translation method. Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction, but before the addition of the secondary d…
Kit for the detection and quantification of apoptotic cell death on a single-cell level by flow cytometry and fluorescence microscopy, and for double labeling with fluorescein-labeled cell markers (TMR red)Benefits Convenient: No secondary detection system required. Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis. Flexible:…
Application The Cellular DNA Fragmentation ELISA may be applied to measure: Apoptosis by detection of BrdU-labeled DNA fragments in the cytoplasm of affected cells (= nonradioactive alternative to the [3H]-thymidine-based DNA-fragmentation assay). Cell-mediated cytotoxicity by detection of BrdU-labeled DNA fragments released from damaged target cells into the culture supernatant (= nonradioactive alternative to the [3H]-thymid…
Application The kit is used for the detection of BrdU incorporated into cellular DNA by immunocyto/histochemistry. Benefits Safe: No radioisotopes are used. Easy to perform: Follows a standard immunohistochemistry protocol. Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU. Flexible: Allows double-labeling protocols.
Application The kit is used for the detection of BrdU incorporated into cellular DNA using immunofluorescence microscopy. It is used for the detection of DNA synthesis by either in vitro labeling of cells or organ cultures, or by in vivo labeling, in which frozen or paraffin-embedded tissue sections must be prepared prior to fixation. Benefits Safe: No radioisotopes are used. Easy to perform: Follows a standard immunof…
Application The Cell Proliferation ELISA, BrdU (colorimetric) belongs to the second, improved generation of kits for measuring DNA synthesis. It is designed as a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell syst…
ApplicationThe In Situ Cell Proliferation Kit, FLUOS belongs to the second generation of improved kits for the detection of DNA synthesis at the single-cell level (in situ) by immunocyto-/immunohistochemistry. The kit can be used in many different in vitro and in vivo cell systems, for example: Detection and quantification of cells in S-phase Determination of growth fractions for tumors in animal-model systems Detectio…
ApplicationThe immunoreactivity of the M30 antibody is confined to the cytoplasm of apoptotic cells. It can be easily used for: Immunocytochemistry Flow Cytometry Immunohistochemistry: paraffin-embedded tissue sections (secondary enhancer reagents needed for this particular application) and cryostat sections Benefits Determine caspase activity, even in formalin-embedded tissue. Identify the stage of apoptosis (…
Quantitative determination of Hu-IFN alpha bioactivity Sample Volume: 25 µl Catalog Number: 51100-1 Quantity: 1 x 96 Assay Range: 1.5-200 IU/ml Shipping Conditions: Dry ice Storage temp.: -80˚C Shelf Life: 9 months from date of manufacture 2007 Retail Price: $895
