Gene Expression and Molecular Cloning Products & Reviews

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Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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T7E1 CRISPR Validation Kit

Merck

The T7 Endonuclease Detection Assay is a well-known method for detecting genome editing events from CRISPR, Zinc-finger nuclease, and TALEN gene targeting. Originally identified from Escherichia coli bacteriophage, the T7 endonuclease can cleave mismatched heteroduplex DNA, Holliday junctions, branched DNA, and cruciform DNA.

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CRISPR Plant

Merck

All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids for use with monocots and dicots.

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Bacterial Gene Editing with CRISPR

Merck

CRISPR/Cas9-mediated recombineering is the most powerful bacterial genome engineering method to date. In addition, Cas9-mediated recombineering overcomes the dependence on a second recombination step, avoids the creation of destabilizing scar sites, can be used in multiplexing, and is less time-consuming than previous protocols.

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CRISPR Vectors and Controls

Merck

Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments. Select the best formats for your mammalian or plant applications: SygRNA ® synthetic gRNA, plasmid DNA, lentiviral particles, and proteins.

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AAV9 Probes

Gator Bio

The Gator ® AAV9 Probes are high specificity nanobody-based biosensors that enable direct capture and quantitation of AAV9 in crude lysates, column eluates, cell lysates and cell culture supernatants. The Gator ® AAV9 probe uses proven CaptureSelect™ (Thermo Fisher Scientific) high affinity and high specificity anti-AAV9 nanobody.

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Cas9 Plus Protein

Merck

Recombinant Cas9 Plus protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.

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Cas9-GFP Protein

Merck

Recombinant Cas9-GFP protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.

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Human CRISPR Brunello Knockout Library

Merck

The human CRISPR ′Brunello′ pooled library is designed using optimized metrics, as published by, Doench et al. Nat Biotechnol. (2016) and described further in Sanson, K.R., et al. Nat Commun (2018)

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Human CRISPR Poison Exon Knockout Library

Merck

The human, paired guide Poison (pgPoison) library targets 3` splice sites with paired guide RNAs for alternative exon removal (pgRNAs), induce skipping of "poison" cassette exons and corresponding upstream constitutive exons in ultraconserved regions.

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Mouse CRISPR Brie Library

Merck

The mouse CRISPR "Brie" lentiviral pooled libraries are designed using optimized metrics, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018), which combine improved on-target activity predictions (Rule Set 2) with an off-target score, the Cutting Frequency Determination (CFD)

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Human CRISPR Activation Calabrese Library

Merck

The Human CRISPR activation library (Calabrese) activates over 18,000 human genes and is used for genome-wide activation screening. The library is designed to be compact and efficient to maximize screening efficiency and performance, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018).

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CRISPRi Control & Effector Kit

Merck

The control kit includes validated positive control targeting RAB1A and non-targeting control for assay set-up and a UCOE KRAB-dCas9 for consistent effector expression across a wide variety of cell lines. The kit is ideal for setting up the screen assay and optimization before using the CRISPRi library pools.

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Human Kinase Lentiviral CRISPR Pool

Merck

This pooled library targets the human kinome, a collection of approximately 700 genes commonly associated with development and disease, with an average of nearly 9 unique knockout CRISPR clones per kinase gene. The lentiviral format allows for stable integration, selection, and enrichment of transduced cells

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