NGS Preparation Kits Products & Reviews

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NGS Preparation Kits

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NEBNext® dsDNA Fragmentase Reaction Buffer

New England Biolabs Inc.

New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction.NEBNext® dsDNA Fragmentase Reaction Buffer Components: 20mM Tris…

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GS Junior Titanium emPCR Kit (Lib-A)

The GS Junior Titanium emPCR Kit (Lib-A) provides reagents for the amplification of an Amplicon library from a single effective (i.e., functionally clonal) bead-bound copy of the DNA template to tens of millions of copies per bead, amenable to sequencing with the GS Junior Instrument using the appropriate GS Junior Titanium kits. One of the main advantages of the emPCR Amplification process is that, while the entire procedure…

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GS Junior Titanium emPCR Kit (Lib-L)

The GS Junior Titanium emPCR Kit (Lib-L) provides reagents for the amplification of a Shotgun, Paired End or cDNA library from a single effective (i.e., functionally clonal) bead-bound copy of the DNA template to tens of millions of copies per bead, amenable to sequencing with the GS Junior Instrument using the appropriate GS Junior Titanium kits. One of the main advantages of the emPCR Amplification process is that, while the…

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GS FLX Titanium emPCR Kits (Lib-A)

Roche

The GS FLX Titanium emPCR Kits (Lib-A) contain all reagents and other components required for two large-volume (LV), medium-volume (MV) or small-volume (SV) emulsion-based clonal amplifications of DNA libraries prepared using the Genome Sequencer System library preparation procedure (Amplicons), for subsequent sequencing on a Genome Sequencer FLX Instrument using the appropriate GS FLX Titanium series Sequencing and PicoTiterP…

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GS FLX Titanium emPCR Kits (Lib-L)

Roche

Kits for large-volume (LV), medium-volume (MV) and small-volume (SV) emulsion-based clonal amplification of a DNA library prepared using the appropriate GS FLX Titanium series library preparation procedure. The GS FLX Titanium emPCR Kits (Lib-L) contain all reagents and other components required for two emulsion-based clonal amplification reactions (which can be pooled, if applicable). Contents The GS FLX Titanium emPCR Kits…

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SureSelect Focused Exome

Agilent Technologies

The SureSelect Focused Exome is a highly targeted design that enables analysis of only disease-associated targets, enabling superior coverage of disease-associated regions even on benchtop sequencers. This design provides 20 or more reads for 95% of targets at 1.5Gb (100x) of sequencing and 98% of targets with 3Gb (200x) of sequencing.

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KAPA Library Quantification Kits

Kapa Biosystems, Inc.

KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of NGS libraries prior to pooling for capture or flow cell amplification. Kits contain KAPA SYBR® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR. The engineered KAPA SYBR FAST DNA Polymerase contained in the Master Mix amplifies GC- and AT-rich DNA fragments of…

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Line-1 Mobilization NGS Assay Kit

Cellecta

Cellecta’s LINE-1 Assay Kit provides a convenient, efficient, and targeted Next-Generation Sequencing (NGS) based approach to identify polymorphic insertion sites of LINE-1 in the human genome. The LINE-1 Assay Kit includes all the components needed to detect and identify novel LINE-1-induced genomic variations in genomic DNA isolated from tumors, blood, cells or other samples. Only a few hundred nanograms of DNA are requir…

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NEXTflex® Dual-Indexed DNA Barcodes

PerkinElmer

The NEXTflex® Dual-Indexed DNA Barcodes are two kits that each contain 96 combinatorial dual indexed barcoded adapters for Illumina® library prep which enable deep multiplexing of NGS sequencing samples compatible with high-throughput automation.They significantly increase scale, while reducing costs by allowing the user to pool up to 192 samples in a single flow cell.

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