PCR and Thermal Cycling Products & Reviews

A comprehensive kit for efficient multiplex RT-qPCR in a single reaction tube. Ideal for multiplex (4-5) assays using dual-labelled hydrolysis probes, works with standard and fast cycling.

A comprehensive kit for performing dye-based RT-qPCR swiftly and reliably in a single tube. No carryover contamination and compatible with standard and fast cycling.

Reliable multiplex qPCR kit. Optimised for amplifying low copy DNA targets. Ideal for multiplexed reactions of 4 or 5 targets without compromising performance.

Ideal for quantification of any DNA template (gDNA, cDNA, viral DNA), amplifies low copy number samples with ease. Utilises our intercalating fluorescent dye, MicroGREEN, that binds to double stranded DNA, making it the perfect choice for qPCR & melt curve analysis.

Swift, cost-effective qPCR detection. Compatible with a wide range of qPCR instruments and mastermixes. Heightened sensitivity and outstanding reproducibility due to high fluorescence. A robust, thermally stable dye endures cycling and freeze-thaw cycles exceptionally well. 

The MegaMix Gold One-step RT-PCR kit contains all the components needed to perform RT-PCR swiftly and reliably. The kit contains all the components needed to perform RT-PCR swiftly and reliably. The kit ensures a highly specific and sensitive amplification, removing background and primer dimer formation. MegaMix Gold boasts excellent accuracy and produces A-tailed products suitable for ligating into TA cloning vectors.

The Micronic Eco-Friendly Choice Rack RM is 99% made of renewed material, setting a new sustainable standard for sample storage racks.

The Temperature-Validation System for the entire Mastercycler family enables simple and rapid validation and calibration of the temperature on the universal blocks of the cyclers. The program for automatic measurement and calibration is integrated into the Mastercycler software, thus furnishing the Mastercycler family with an accurate and precise system that meets all documentation and detection requirements in the laboratory.

The Eppendorf CycleManager is a Windows based software for programming and controlling one or more Mastercyclers. The thourough databank ensures reliable storage of all GLP relevant experimental data incl. detailed user comments.The main dialogue box is the central user interface. All key functions can be accessed from it. Mastercyclers can be added or removed from the Multi-Cycler-Network. It offers also an overview of all co…

• Strips with 8 microcaps for 0.2 ml wells• Easy and rapid sealing of Eppendorf twin.tec plates and other standard microtiter plates, see also Capping Aid• Easy to remove using extension at the end of the strip• Autoclavable (121°C, 20 min.)• Certified free of human DNA, DNase, RNase and PCR Inhibitor**Certificate, test procedures and detailed information on request.

Rack for handling and storing tubes and plates For 0.2 ml, 0.3 ml, 0.5 ml tubes and 96-well microtiter plates Stackable Can be stored down to -90°C Autoclavable (121°C, 20 min.)

The CpG WIZ® p16 Amplification Kit is used for determining the methylation status of the p16 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified such that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and unmethylated)…

The CpG WIZ® p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the p15 promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify template under the same conditions. Control genomic DNA samples (methylated and unm…

The CpG WIZ® E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and…

The CpG WIZ® Prader-Willi/Angelman Amplification Kit is used for determining the methylation status of this region by methylation -specific PCR (MSP). The kit contains primers targeted to the CpG island on the SNRPN gene. PCR parameters have been identified such that both primer sets in the kit amplify template under the same conditions. Normal control genomic DNA, which will generate a product with both primer sets, is also i…

CpGenome™ Universal Unmethylated DNA Set provides 2 separate genomic DNA controls for gene methylation studies. This product is intended for use with the CpGenome™ DNA Modification Kit (S7820) and can be subsequently used as an unmethylated DNA control for gene methylation studies.

The CpGenome™ DNA Modification Kit (Cat. No. S7820) contains the reagents necessary to perform the initial bisulfite reactions, while the CpG WIZ® BRCA1 Amplification Kit contains the reagents required for the PCR amplification reactions.

With a uniform thermal ramp rate of over 10°C per second xxpress™ is the fastest real-time thermocycler in the World delivering 40 cycle q-PCR in under 10 minutes. And whilst not all PCR chemistries are yet as fast as this system there are a number that repeatably and reliably work at this speed. Ultra low thermal mass and high conductively enable the patented resistive heating system deliver this speed cycle more efficiently…

An arrayed or non-arrayed BAC library is the preferred choice for downstream applications such as hybridization screening; full-genome sequencing; BAC end sequencing or physical mapping projects. Amount of clones received is based on a guaranteed coverage and 120Kb average insert size. HMW DNA isolation and QC by HMW DNA enzymatic digestion are included. Deliverables for ARRAYED BAC library: Clones will be arrayed and amplifie…

A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive. We typically construct a minimum 5X BAC library and BAC clones are then pooled and distributed in 96-well plates. Each well will contain about 400-500 original BAC clones. HMW DNA isolati…

Our uncloned normalized cDNA is created using proprietary technology which features full-length-enriched and high quality size-fractionated cDNA. Our cDNA has been used successfully with FLX, Titanium an Illumina sequencing.

Our cDNA libraries feature full-length-enriched and high quality size-fractionated cDNA. cDNA library construction is usually done in slightly-modified versions of pBluescript or pcDNA3.1. Insert sizes will range from about 0.5Kb to 4Kb. Concerning QC steps, we evaluate the average insert size, empty clone rate and clone concentration/tube before shipping out. We always provide a gel picture of randomly selected clones showing…

ASH is APA-based, full-length-enriched, single-stranded cDNA subtraction hybridization library. - ASH cDNA subtraction is full-length-enriched and independent of PCR suppression. - ASH cloning is directional and does not involve normalization. - ASH method provides non-exponential amplification before subtraction because double-stranded cDNA is not required. - ASH method has a better subtraction effect because 1) a high ratio…

RealTime ready Custom RT-qPCR Assays and Panels Get a head start on your gene expression analysis with function tested assays for all annotated human, mouse, and rat genes Achieve high specificity using intron-spanning assays based on short hydrolysis LNA-substituted Universal ProbeLibrary (UPL) Probes.  Rely on stringently RT-qPCR-tested assays that are compliant with MIQE (Minimum Information for Publication of Quantitati…

2 user licenses, data analysis software for all CFX real-time PCR detection systems, 2 HASP HL keys, calibration kit, installation CD