UHPLC and HPLC Products & Reviews

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High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Guard Cartridge Holders

Thermo Fisher Scientific

The Direct Connection UNIGUARD allows the guard cartridge outlet to be directly placed against the analytical column inlet frit. Product detail: We recommend all analytical columns are protected by a guard cartridge, which is replaced on a regular basis. The ID of the analytical column determines the correct ID for the guard cartridge. We offer 10x1, 10x2, 10x3, 10x4mm guard cartridges in all our stationary phases to ensur…

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Javelin Guard Columns

Thermo Fisher Scientific

The Javelin guard design is a one-piece format that can be installed without wrenches and requires no holder, which allows quick and easy replacement. Product detail: We recommend all analytical columns are protected by a guard column or cartridge, which is replaced on a regular basis. The ID of the analytical column determines the correct ID for the guard, so we offer 1.0, 2.1, 3.0 and 4.0 diameter Javelin guard columns in…

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HyperREZ™ Polymeric Carbohydrate Columns

Thermo Fisher Scientific

HyperREZ™ columns are specially designed for analysis of organic acids, alcohols, saccharides and carbohydrates. They employ a polymeric support with ligand exchange groups. The ligand exchange resins contain highly sulfonated cation exchange resins that have Group I, Group II or transition metals associated with the chromatographic surface.HyperREZ™ columns are based upon a polymeric resin that shows stability from pH 1 to 14…

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BioBasic 8/4/AX/SCX/18 Columns

Thermo Fisher Scientific

BioBasic 8 Columnso   Improved reproducibility, efficiency and column lifetimeso   Range of stationary phases to optimize selectivityo   Powerful resolution, even on short column lengths Product detail:BioBasic 8 columns provide exceptional reliability for separations of proteins, peptides, and other biomolecules through a combination of high purity silica and reproducible, stable bonding.  The proprietary bonding procedure en…

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BetaMax Acid/Neutral/Base Columns

Thermo Fisher Scientific

BetaMax Acid Columns o   High surface area area and bonded phase coverage for maximum retention, even in high percentage organic conditionso   Chemistries for acidic compounds Product detail: For alternative selectivity to traditional alkyl phases, we recommend you try the polar embedded phase BetaMax columns. The stationary phase contains urea groups embedded within the C12 chain, which extra interactions with samples to…

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BETASIL C18/C6/D101/C1/CN Columns

Thermo Fisher Scientific

BETASIL C18 Columnso   Broad range of particle sizes and phases for wide method development optionso   High surface area and phase coverage for reproducibility and sharp peak shapes Product Detail BETASIL columns are based on 100Å pore size, fully deactivated type B silica to ensure sharp peaks and excellent reproducibility for the analysis of a wide range of compounds. The 100Å pore size gives a higher concentration of statio…

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BetaBasic 4/8/18 Columns

Thermo Fisher Scientific

BetaBasic 4 Columnso   Excellent peak shapes for basic compoundso   Superb stability at both low and high pH Product detail:BetaBasic 4 columns are based on a 150Å pore size, fully deactivated type B pure silica to ensure sharp peaks and excellent reproducibility for the analysis of basic compounds.  Due to stable bonding chemistry and excellent surface coverage, BetaBasic 4 columns show excellent peak shape for basic compound…

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Hypercarb Columns

Thermo Fisher Scientific

o   100% porous graphitic carbon with very different selectivity to reversed-phase silica columnso   Exceptional retention of very polar compoundso   Separation of structurally similar compoundso   Stable from pH 1 to 14 at high temperatures in a wide range of solvents Product detail: Hypercarb is a unique material for HPLC manufactured by Thermo Hypersil-Keystone. Hypercarb consists of fully porous spherical carbon particle…

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Hypersil Duet C18/SCX Columns

Thermo Fisher Scientific

o  Separate hydrophobic and ionic compounds in a single runo  Select from two mixed mode phases, using either cation or anion exchange combined with C18o  Retention of compounds is optimized using mobile phase pH, ionic strength and percent organic Product detail: Hypersil Duet columns are an ideal choice for unknown compounds and metabolism studies, as they can separate hydrophobic and ionic compounds in a single analysis…

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Hypersil SAX Columns

Thermo Fisher Scientific

The Hypersil SAX is recommended for analysis of small organic molecules and organic acids. Product detail: Hypersil SAX is a strong anion exchange column, which is typically used for  analysis of small organic molecules and organic acids. As the columns are based on the first generation classical 120Å pore size silica they are not recommended for analysis of biomolecules. For new methods we recommend trying our latest ion…

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Column Heater and Chiller Units From Thermo Electron

Thermo Fisher Scientific

Thermo Electron Hot and Cool Pockets™ offer unbeatable value in coulmn temperature control. The pockets are small and light weight yet offer precise temperature control. Hot pockerts heat from 5 to 85 oC with a temperature accuracy of ±2oC over the entir range, and a temperature stability of ±0.1Oc. Hot Pockets™ and Cool Pockets™ are not available within the European Union at the present time. We apologise for any inconvenien…

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8-SC(B)

Chromacol Ltd

A new screw cap that has been introduced for HP users, to enable them to use a screw top vial on a robotic arm autosampler.

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PRP-X100

Hamilton Company

Easy Ion DeterminationHamilton PRP-X100 ion chromatography columns are the easy way to separate inorganic ions. You can use these columns with any existing HPLC or ion chromatograph to easily determine anions in almost any sample matrix including: air, water and soil. PRP-X100 columns provide a number of benefits.Better than Wet Chemical MethodsAnyone using wet chemical or colorimetric methods will find that ion chromatography…

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Hamilton RCX-10 And RCX-30 Columns

Hamilton Company

Hamilton RCX-10 And RCX-30 Columns for the Anion Exchange Separation of CarbohydratesHamilton RCX-10 carbohydrate analysis columns are designed for the isocratic and gradient separation of carbohydrates. Samples containing two or three carbohydrates, for example Ice Cream can be quickly separated isocratically while more complex samples like Corn Syrup, require gradient elution to fully resolve all the oligomers (DP1 to DP7).…

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HC-75

Hamilton Company

Separate Mono and Disaccharides or Carbohydrate Oligomers from DP1 to DP8.Hamilton HC-75 columns separate carbohydrates by ligand exchange and size exclusion mechanisms.HC-75 is a more highly crosslinked gel (7.5%) that utilizes ligand exchange to separate carbohydrates. Calcium, Hydrogen and Lead are available as counter ions. The calcium form column is more commonly used because of its broader applicability.For application e…

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Agilent Pursuit XRs Diphenyl HPLC Columns

Agilent Technologies

100Å pore size, high ligand density;  available in 1.0, 2.0,  3.0, 4.6 and 10 mm ids;  30 - 250 mm lengths. Pursuit columns are ideal for pharmaceutical applications using LC/MS, from analyzing lead compounds and biological samples in drug discovery, to implementing dependable analysis of raw materials and approved drugs in the QC process.  Build on the large 200A pore size silica, high liquid density delivers up to 40% faster…

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Astec CHIROBIOTIC™ V2 Chiral HPLC Column

Sigma-Aldrich Supelco

The polar organic and the polar ionic modes have become increasingly popular largely because of their ideal compatibility with MS detection and preparative LC. Two years of extensive research at Astec in understanding these mechanisms has resulted in new possibilities for extending their effectiveness even further. Important changes in linkage chemistries and the silica and the silica have now led to the new CHIROBIOTIC V2 and…

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Agilent 1290 Infinity II Evaporative Light Scattering Detector

Agilent Technologies

The 1290 Infinity II Evaporative Light Scattering Detector (ELSD) is ideal for reproducible, sensitve detection of both volatile and nonvolatile compounds that cannot be detected by UV. A unique peltier cooled evaporator coupled with an original gas flow design allow subambient operation down to 10 °C. This delivers maximum sensitivity for compounds with significant volatility below 30 °C.

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Midi HPCCC range

Dynamic Extractions

The DE Midi range of High Performance CCC (HPCCC) equipment is based on traditional liquid/liquid partitioning and extends the purification options available to chemists, primarily in Development, who are looking purify samples from 5 grams to 1,000 grams of material. The equipment is easily connected and interfaced into existing LC equipment and provides options when existing solid phase technology cannot obtain the resolutio…

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Solvent Safety Products

Kromatek

SCAT solvent safety systems have been designed to bring solvent safety to the laboratory. The specially designed caps for both solvent reservoirs and waste containers fit virtually any sized container. Certified by TUV in Germany these safety systems af guaranteed to protect your health in the laboratory. Recent legislation has meant the use of solvent safety systems are mandatory in a number of EU countries and several EU m…

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PSS GPC and SEC

Kromatek

With a fresh and customer focussed approach to Polymer Analysis, PSS is Germany's leading authority in Gel Permeation Chromatography, GPC and SEC. The most comprehensive range of products for standards, columns instruments and software coupled with more than twenty years experience in delivering customer oriented results. A complete range of standards for organic and aqueous GPC Columns for organic separations Columns f…

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Evaporative Light Scattering Detectors

Kromatek

ELSD detectors - 3 models to choose from Model 100 ELSD – Simple OperationModel 300S ELSD – Advanced ControlModel 400 ELSD – Sophisticated Sytem Management Highest dynamic range 4.5 orders of magnitude Equal response between analytes High sensitivity even for semi-volatiles Independantly control the temperature of the nebuliser and drift tube

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Chromatography Data System

Kromatek

AZUR 4.0 The latest version of chromatography data system for stand-alone acquisition and analysis. Many new features including enhanced reporting capabilities and the new 24 bit USB interface. USB or PCI interface up to 4 independant channels Simple user interface Advanced functionality Low cost academic version

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