UHPLC and HPLC Products & Reviews

High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Thermo Scientific™ Vanquish™ Dual Pump F

Thermo Fisher Scientific

Obtain maximum application flexibility including Dual LC, Tandem LC or LC-MS, and Inverse Gradient workflows using the biocompatible Thermo Scientific™ Vanquish™ Dual Pump F. This pump provides two independent ternary-solvent blending flow streams in one housing. Our built-in Thermo Scientific™ SmartFlow™ pumping technology automatically compensates for changing eluent compressibility ensuring excellent flow and gradient preci…

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Thermo Scientific™ UltiMate™ LPG-3400XRS Extended Pressure Range Quaternary Pump

Thermo Fisher Scientific

Enjoy the flexibility of a quaternary pump with the fast gradient performance of a binary pump. The unique Thermo Scientific™ UltiMate™ LPG-3400XRS Extended Pressure Range Quaternary Pump is an LC/MS optimized quaternary UHPLC pump offering state-of-the-art performance for high speed and ultrahigh resolution applications. The compact pump head design, with a gradient delay volume of just 200µL, supports ballistic gradients for…

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Thermo Scientific™ Vanquish™ Quaternary Pump F

Thermo Fisher Scientific

Extend the capabilities of the Thermo Scientific™ Vanquish™ UHPLC platform by adding all the flexibility you can expect from a state-of-the-art quaternary solvent blending. The biocompatible Thermo Scientific™ Vanquish™ Quaternary Pump F incorporates our SmartFlow™ pumping technology, which ensures excellent flow and gradient precision, independent of eluent composition and backpressures up to 1034 bar. This gives you unrivale…

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Thermo Scientific™ UltiMate™ HPG-3400RS Rapid Separation Binary Pump

Thermo Fisher Scientific

For more mobile phase flexibility in any ultrahigh speed and ultrahigh resolution application, the Thermo Scientific™ UltiMate™ HPG-3400RS Rapid Separation Binary Pump offers more solvent capability. With an unrivaled flow-pressure footprint, the RSLC pump is the most flexible binary UHPLC pump available. The UltiMate 3000 Binary RS pumps are capable of providing true ballistic gradients with excellent retention time precision…

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Thermo Scientific™ UltiMate™ HPG-3200RS Rapid Separation Binary Pump

Thermo Fisher Scientific

With exceptional flow stability and adjustable delay volume, the Thermo Scientific™ UltiMate™ HPG-3200RS Rapid Separation Binary Pump excels at high-speed and high-resolution applications. An industry-leading flow/pressure footprint of 100 MPa (15,000 psi) at up to 5mL/min. and 80 MPa (11,600 psi) at 8mL/min. makes it the most flexible binary pump choice. Designed with a stainless-steel-free (biocompatible) flow path, the HPG-…

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Thermo Scientific™ UltiMate™ HPG-3200SD Standard Binary Pump

Thermo Fisher Scientific

Enjoy accurate gradients and precise retention times while meeting the requirements of diverse sample types with a wide range of flow rates. The Thermo Scientific™ UltiMate™ HPG-3200SD Standard Binary Pump is ideal for analytical LC separations on standard-bore columns. With support for pressures up to 62MPa (9,000 psi, 620 bar) at flow rates of up to 10 mL/min., UltiMate 3000 SD pumps excel in standard LC applications offer a…

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Thermo Scientific™ Vanquish™ Binary Pump H

Thermo Fisher Scientific

Developed to offer more pressure capabilities than ever before, the Thermo Scientific™ Vanquish™ Binary Pump H comes without any tradeoff on durability and robustness. From ultrafast to extremely shallow gradients at pressures up to 150 MPa, the industry-leading fourth generation Thermo Scientific™ SmartFlow™ pumping technology of the Vanquish system always provides you with unmatched retention time reproducibility and the low…

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Thermo Scientific Accucore Biphenyl LC column

Thermo Fisher Scientific

Having trouble resolving your compounds using your C18 column? The new Thermo Scientific Accucore Biphenyl LC column is your solution for: Aromatic & moderately polar analytes, Drugs of abuse, Steroids Now you can stop struggling with unresolved peaks, long run times, and poor lot-to-lot consistency. Obtain unique selectivity for aromatic and moderately polar analytes using Accucore Biphenyl LC Columns. These columns have opti…

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Thermo Scientific™ ProPac™ Elite WCX

Thermo Fisher Scientific

Discover the unique Thermo Scientific™ ProPac™ Elite WCX weak cation exchange (WCX) liquid chromatography column. Designed for protein characterization including therapeutic proteins, monoclonal antibodies (mAbs), antibody drug conjugates (ADCs) and biosimilar proteins, this column offers high resolution, high efficiency separation of proteins and their charge variants. The unique column chemistry provides excellent perform…

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Hypercarb PREP HPLC Columns

Thermo Fisher Scientific

Porous graphitic carbon (PGC, Hypercarb™) has unique properties as a stationary phase in high-performance liquid chromatography (HPLC). Its chemical surface properties distinguish it from more conventional LC packings such as bonded-silica gels and polymers. PGC behaves as a strongly retentive alkyl-bonded silica gel for non-polar analytes. However, its retention and selectivity behavior towards polar and structurally-related…

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