Immunoassays Products & Reviews

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Antibody development

BBI Solutions

Our unique combination of custom antibody development, lateral flow  development and manufacturing services, signal enhancement technology and our mobile diagnostic platform are shaping the future of lateral flow. Our partnership approach allows you to work with one company to reach your assay goals.

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IP-One ELISA assay kit

Cisbio Bioassays

IP-One ELISA assay kit from Cisbio Bioassays - Simple and highly accurate measurement of IP1 IP1, a downstream metabolite of IP3, accumulates in cells following activation of Phospholipase C (PLC) coupled receptors. Among them, the Gq coupled GPCRs represent the most important family of receptors which can activate the β subtype of the PLC family. Other receptor types, like protein tyrosine kinase receptors, antigen or immuno…

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Annexin-V-Biotin

Biotin-conjugated anticoagulant for the detection of phosphatidylserine in the outer leaflet of apoptotic cellsProduct Description Detection: Annexin-V-Biotin can be used in immunohistochemistry with a secondary detection system (e.g., Streptavidin-POD conjugate). Sample material: Cell lines and freshly isolated cells Specificity: Annexin-V binds in a calcium-dependent manner to negatively charged phospholipid surfaces, and…

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Cell Death Detection ELISA

Roche Cell Death Detection ELISA - Photometric enzyme immunoassay for the quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) after induced cell deathBenefits of Roche Cell Death ELISA:  High sensitivity: (5 x 102 cells/ml) Fast performance: (5 - 6 hours) No prelabeling of cells necessary Nonradioactive assay system No species restrictions Easy handling…

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In Situ Cell Death Detection Kit, POD

Kit for the detection and quantification of apoptotic cell death on a single-cell level by light microscopy in immunocyto- and immunohistochemistry Benefits Sensitive: The maximum intensity of labeling (cell staining) of apoptotic cells is higher than the nick translation method. Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction, but before the addition of the secondary d…

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In Situ Cell Death Detection Kit, TMR red

Kit for the detection and quantification of apoptotic cell death on a single-cell level by flow cytometry and fluorescence microscopy, and for double labeling with fluorescein-labeled cell markers (TMR red)Benefits Convenient: No secondary detection system required. Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis. Flexible:…

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Cellular DNA Fragmentation ELISA

Application The Cellular DNA Fragmentation ELISA may be applied to measure: Apoptosis by detection of BrdU-labeled DNA fragments in the cytoplasm of affected cells (= nonradioactive alternative to the [3H]-thymidine-based DNA-fragmentation assay). Cell-mediated cytotoxicity by detection of BrdU-labeled DNA fragments released from damaged target cells into the culture supernatant (= nonradioactive alternative to the [3H]-thymid…

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5-Bromo-2'-deoxy-uridine Labeling and Detection Kit II

Application The kit is used for the detection of BrdU incorporated into cellular DNA by immunocyto/histochemistry. Benefits Safe: No radioisotopes are used. Easy to perform: Follows a standard immunohistochemistry protocol. Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU. Flexible: Allows double-labeling protocols.

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5-Bromo-2'-deoxy-uridine Labeling and Detection Kit I

Application The kit is used for the detection of BrdU incorporated into cellular DNA using immunofluorescence microscopy. It is used for the detection of DNA synthesis by either in vitro labeling of cells or organ cultures, or by in vivo labeling, in which frozen or paraffin-embedded tissue sections must be prepared prior to fixation. Benefits Safe: No radioisotopes are used. Easy to perform: Follows a standard immunof…

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Cell Proliferation ELISA, BrdU (colorimetric)

Application The Cell Proliferation ELISA, BrdU (colorimetric) belongs to the second, improved generation of kits for measuring DNA synthesis. It is designed as a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell syst…

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In Situ Cell Proliferation Kit, FLUOS

ApplicationThe In Situ Cell Proliferation Kit, FLUOS belongs to the second generation of improved kits for the detection of DNA synthesis at the single-cell level (in situ) by immunocyto-/immunohistochemistry. The kit can be used in many different in vitro and in vivo cell systems, for example: Detection and quantification of cells in S-phase Determination of growth fractions for tumors in animal-model systems Detectio…

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M30 CytoDEATH, Fluorescein

ApplicationThe immunoreactivity of the M30 antibody is confined to the cytoplasm of apoptotic cells. It can be easily used for: Immunocytochemistry Flow Cytometry Immunohistochemistry: paraffin-embedded tissue sections (secondary enhancer reagents needed for this particular application) and cryostat sections Benefits Determine caspase activity, even in formalin-embedded tissue. Identify the stage of apoptosis (…

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iLite Human Interferon Alpha kit

PBL InterferonSource

Quantitative determination of Hu-IFN alpha bioactivity Sample Volume: 25 µl Catalog Number: 51100-1 Quantity: 1 x 96 Assay Range: 1.5-200 IU/ml Shipping Conditions: Dry ice Storage temp.: -80˚C Shelf Life: 9 months from date of manufacture 2007 Retail Price: $895

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SIRT1

Cisbio Bioassays

An acetyl-biotinylated substrate recognized by a Eu3+ anti-acetyl MAb and SA-XL665, which is used in activator screening or to test an High Throughput SIRT1 inhibitor.  The level of enzymatic activity is inversely proportional to the amount of signal produced in the system. For more information please visit Cisbio Bioassays website.

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Iso-Gold™ Rapid Mouse-Monoclonal Isotyping Kit

BioAssay Works, LLC

The Iso-Gold kit is a rapid-format isotyping kit that reduces the labor of the traditional ELISA kit from a day-and-a-half procedure to a five-minute assay. It determines the isotype of your monoclonal antibody, assess the purity of your purified monoclonal antibody*, verify the purity of your purchased monclonal antibody* and reduces runtime. *multiple lines appear and indicate subclass contamination

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Osteoprotegerin EIA

Osteoprotegerin (OPG) EIA is an enzyme immunoassay intended for the quantitative determination of OPG in plasma, serum or cell culture supernatants. OPG is a dimeric glycoprotein of the tumor necrosis factor (TNF) receptor family that acts as a soluble receptor for sRANKL (soluble receptor-activated NF-kB ligand) and prevents it from binding to and activating RANK, thus inhibiting osteoclast development. Possible indications f…

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sRANKL (Free) Ultrasensitive EIA

The Ampli-sRANKL EIA is an enzyme immunoassay intended for the quantitative determination of human free soluble RANK-Ligand in plasma, serum and cell culture supernatants. Format:ELISA / EIA, Sample Type:Serum, Plasma, Cell Culture, Sample Size:100 µl, Size:96 Wells, Range:0.125 - 2 pmol/l, Sensitivity:0.02 pmol/l, Incubation Time:Overnight, 1 hour 30 minutes

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Oxidized LDL (Ab) EIA

Oxidized LDL (Ab) EIA is a enzyme immunoassay intended for the quantitative determination of human IgG autoantibodies to oxidized low density lipoprotein (LDL) in serum. Format:ELISA / EIA, Sample Type:Serum, Sample Size:50 µl, Size:96 Wells, Range:37 - 1200 mU/ml, Sensitivity:27 mU/ml, Incubation Time:2 hours 20 minutes

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