Real-Time Reverse Transcription PCR (qRT-PCR) Mastermixes and Kits Products & Reviews

The High Capacity cDNA Reverse Transcription Kit contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream appli…

Brilliant II SYBR qPCR and QRT-PCR reagents provide improved sensitivity and detection while ensuring reproducible quantification at low target concentrations. Compared to other commercially available qPCR reagents, Brilliant II SYBR qPCR and QRT-PCR reagents have earlier threshold cycle (Ct) detection at high and low template concentrations.

Brilliant III qPCR and QRT-PCRE Master Mixes are more sensitive and faster via the quick-activating hot start and the engineered Taq-mutant. Brilliant III ultrafast maintains amplification efficiency, R2, dynamic range, and detection sensitivity. Shorter total cycle time.

Alien Reference RNA QRT-PCR Detection Kit for Monitoring the Overall Performance of QRT-PCR Assays.  High-quality external control for detecting inhibitors in RNA samples  Highly sensitive to various inhibitors  No significant homology to any known sequences  Ideally suited for assay standardization applications

qPCRBIO Probe 1-Step Go is a universal probe kit designed for fast, highly specific and ultra-sensitive probe-based real-time RT-PCR.

The GoTaq®Real-Time PCR Systems are ready-to-use 2X master mixes optimized for quantitative PCR in dye (using BRYT(R) Green Dye) or hydrolysis probe detection format.   The 1-Step and 2-Step RT-qPCR Master Mixes contain GoScript™ Reverse Transcriptase for efficient first-strand cDNA synthesis.     GoTaq Real-Time PCR Systems Features & Benefits: Higher fluorescence, often resulting in earlier Cqs Direct su…

The GoTaq® Probe 1-Step RT-qPCR System is optimized for quantitative PCR assays in the hydrolysis probe detection format. The system enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® Probe qPCR Master Mix in single-step real-time amplification reactions. The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimi…

The TaqMan® One-Step RT-PCR Master Mix Reagents Kit, which combines the reverse transcription and amplification steps, permits excellent results even when quantifying minute amounts of RNA. Set-up is quick, easy, and minimizes risk of sample crossover and contamination, making this kit well-suited for all high-throughput applications. • Optimized for high sensitivity and yield • Combined RT (reverse transcription) and amplific…

SensiFAST™ Probe Hi-ROX Kit and SYBR® Hi-ROX Kit have been developed for fast, highly reproducible real-time PCR and has been validated on commonly used real-time PCR instruments. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that SensiFAST Kits delivers fast, highly-specific and ultra-sensitive real-time PCR.SensiFAST™ Probe Hi-ROX Kit has been d…

The Takara Bio TB Green Premix Ex Taq (Tli RNase H Plus), an intercalating dye-based qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications including gene expression analyses, genotyping, and high-throughput studies.

For Greater Specificity, Sensitivity & Yields in cDNA Synthesis and qPCR in a single tube

igScript™ one step RT-qPCR kit combines two powerful mixtures: i). igScript™ Reverse Transcriptase and ii) ig SYBR® Green qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity, and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions. igScript™ Reverse Transcriptase is a recombinant MMLV r…

Even with challenging RNA samples, you can get good results quickly and easily. The Invitrogen SuperScript IV One-Step RT-PCR System combines high-processivity Invitrogen SuperScript IV Reverse Transcriptase (RT) and high-fidelity Invitrogen Platinum SuperFi DNA Polymerase to provide super one-step RT-PCR performance.

FastStart Universal Probe Master (Rox) includes a novel reference dye, which enables its use without modification or adjustments to the specific instrument or protocol on all real-time PCR instruments requiring normalization with ROX. This ready-to-use, 2x-concentrated master mix contains all reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, including qPCR and two-s…

The Power SYBR® Green RNA-to-CT™ 1-Step Kit provides high sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction kit. A novel formulation helps reduce false-positive results caused by primer-dimers that are common in SYBR® Green-based assays. This significantly improves data accuracy and enables the reliable detection of low expressing targets or targets present at low amounts. Minimized hands-on time wit…

The SYBR Green RT-PCR Kit provides all the necessary components to perform both one- and two-step RT-PCR, which makes it ideal for evaluation between the two RT-PCR protocols.

GoTaq®2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method. The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA inp…

Qualified reagents for the polyadenylation of microRNAs (miRNAs) from total RNA and synthesis of first-strand cDNA from the tailed miRNAs.

Brilliant II QRT-PCR reagents were developed to maintain the high quality of our original Brilliant product line, but with a significant improvement in sensitivity of detection, ensuring reproducible quantification even at low target concentrations. With the addition of ROX reference dye to our master mix, we have streamlined the reaction setup process.

100 x 50 µl reactions, real-time PCR mix, 2x mix contains dNTPs, 50 U/ml iTaq™ DNA polymerase, 6 mM MgCl2, SYBR® Green I, 20 nM fluorescein

Ambion® Cells-to-CT™ Kits allow you to measure relative gene expression by real-time RT-PCR without having to purify RNA prior to amplification. Cells-to-CT™ Kits are available for both TaqMan® and SYBR® Green detection. Cells-to-CT™ kits provide a complete workflow for real-time qRT-PCR analysis directly from cultured cells without RNA purification. Featuring a unique method for lysing cultured cells while removing genomic…

Appropriate for COVID-19 detection.

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations. The high RT efficiency of SuperScript III RT and the optimized formulation allow the mast…

Alien Reference RNA QRT-PCR Detection Kit for Monitoring the Overall Performance of QRT-PCR Assays.

Alien Reference RNA QRT-PCR Detection Kit for Monitoring the Overall Performance of QRT-PCR Assays.