Air-Dryable Inhibitor-Tolerant Probe Mix
Air-Dryable Inhibitor-Tolerant Probe Mix is a glycerol-free 4x qPCR mix that combines our novel inhibitor-tolerant chemistry alongside our cutting-edge air-dryable technology. Now you can benefit from dried, room-temperature stable qPCR assays capable of highly-sensitive DNA detection on the most challenging, inhibitor-rich sample types.
Dry qPCR assays in as little as 80 minutes
No loss of activity after 12 weeks at 37 °C
Add only primers & probes before drying
Receive your quote directly from the manufacturer.
Air-Dryable Inhibitor-Tolerant Probe Mix includes hot start Taq DNA polymerase, magnesium, dNTPs and a blend of excipients to ensure reliable drying without loss of activity. The mix is compatible with all probe chemistries, including TaqMan, Scorpions and molecular beacons, and can be used with all commercial real-time PCR instruments. The mix can by dried down in a standard laboratory oven for long term storage and is intended for use in in vitro diagnostic (IVD) kit development, or by anyone wishing to benefit from the advantages of dried qPCR reactions.
The mix is tolerant to a broad range of common PCR inhibitors, including: standard laboratory chemicals (SDS, guanidine, and ethanol) and biological sample inhibitors, such as those found in blood samples (hemin, hematin, haemoglobin, heparin, immunoglobulins (IgGs), lactoferrin, sodium citrate), saliva, urine (urea), plant, and environmental samples (humic acid, catechin, quercetin, tannic acid, cellulose, and chlorophyl). The mix has been extensively validated against crude (or diluted) saliva and blood, along with multiple other pure chemical inhibitors.
Features
- Broad-spectrum inhibitor tolerance
- Sensitive detection of DNA, 4 copies per reaction
- Easy reaction set up, add only primers & probes before drying
- Rapid air-drying protocol, 80-90 min drying time
- Same high performance before & after drying
- Room temperature storage and shipping of dried reactions
- Standard and ultra-fast cycling (down to 3 min RT reaction and 1 s denaturation, 3 s annealing/extension for PCR)
Key benefits of air-drying
- Reduced volume and mass of reaction mixes for cheaper shipping and storage
- Room temperature stability of the air-dried final gel eliminates the need for cold-chain shipping and storage. This simplifies logistics and reduces overall cost.
- Increased volumes of sample can be used. The sample input volume can equal the final reaction volume. This is perfect for dilute samples (with a low target copy number).
- Air-drying in an oven is a highly accessible method, because there is no requirement for specialist equipment and knowledge, eliminating the need for additional staff or project out-sourcing
- Increased control over the drying process and final product development for small and medium sized IVD kit developers
