UltraScript® cDNA Synthesis Kit Separate Oligos
UltraScript® cDNA Synthesis Kit Separate Oligos contains all the components for efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations. Anchored oligo(dT) and random hexamers are provided separately for user optimisation depending on the type of analysis needed.
Reliable and versatile reverse transcription
Unbiased representation of mRNA ends
Broad dynamic range of reverse transcription
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UltraScript® cDNA Synthesis Kit Separate Oligos includes UltraScript® Reverse Transcriptase, an optimised buffer, and separate tubes of anchored oligo(dT) and random hexamers, for user optimisation depending on your downstream application.
UltraScript® Reverse Transcriptase is a thermostable and extremely active modified MMLV reverse transcriptase. The reaction temperature can be increased up to 55 °C providing higher specificity and efficient transcription of GC-rich RNA regions with a high secondary structure. The enzyme is blended with an advanced RNase inhibitor preventing degradation of RNA by contaminating RNase. UltraScript® Reverse Transcriptase is not inhibited by ribosomal and transfer RNAs, making total RNA an ideal substrate.
Features
- Reduced RNase H activity
- Thermostable reverse transcriptase 45 °C to 55 °C
- Advanced RNase inhibitor
- High cDNA yields from as little as 4 pg total RNA
- Accurate reverse transcription of GC-rich templates
- Sensitive detection of low copy number transcripts
- Advanced buffer chemistry including Mg and dNTPs
- Both premixed or separate oligo cDNA synthesis kits available
