UltraScript® 2.0 cDNA Synthesis Kit Separate Oligos
UltraScript 2.0 cDNA Synthesis Kit Separate Oligos containins all the components required for fast, reliable and unbiased cDNA synthesis of the most challenging RNA sample types. Anchored oligo(dT) and random hexamers are provided separately for user optimisation depending on the type of analysis needed.
Thermostable cDNA synthesis kit
Higher activity across elevated temperatures
Increased upper limit of RNA input
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The enhanced thermostability of UltraScript® 2.0 cDNA Synthesis Kits allow reaction temperatures of over 55 °C to be used, giving improved specificity, higher cDNA yields, and more full length cDNA product. The kits use UltraScript 2.0 Reverse Transcriptase which remains partially active even up to 90 °C and is designed for efficient reverse transcription of the most difficult RNA templates, including GC-rich and highly structured transcripts.
UltraScript® 2.0 Reverse Transcriptase is designed for cDNA synthesis from a broad range of RNA concentrations. These kits can be used with 20 pg to 3.5 μg total RNA or oligo(dT) purified mRNA. The RTase is blended with an advanced RNase inhibitor preventing degradation of RNA by contaminating RNase.
UltraScript® 2.0 Reverse Transcriptase is available in three convenient formats, each with dNTPs, Mg, and RNase inhibitor included. UltraScript® 2.0 cDNA Synthesis Kit Separate Oligos provides anchored oligo(dT) and random hexamers in separate tubes, for user optimisation depending on the type of analysis needed.
Features
- Highly thermostable reverse transcriptase 55 °C to 65 °C and above
Advanced RNase inhibitor - High cDNA yields from as little as 20 pg total RNA
- Accurate reverse transcription of GC-rich and highly structured transcripts
- High sensitivity for challenging templates
- Reduced RNase H activity
- Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos
