Clara® Inhibitor-Tolerant Probe 1-Step Mix

PCR and qPCR can be inhibited by a wide variety of chemical compounds, negatively affecting qPCR workflows and diagnostic processes. Clara^® Inhibitor-Tolerant Probe 1-Step Mix is a 4x reaction-ready Clara^® mix combined with our novel inhibitor-tolerant chemisty. It includes a hot start DNA polymerase, UltraScript^® Reverse Transcriptase, RNase inhibitor, dNTPs and magnesium in a single-tube format. The mix can be used to amplify both DNA and RNA samples without a loss in efficiency, regardless of template type.

Clara^® Inhibitor-Tolerant Mixes have been extensively engineered for use in crude saliva testing but tolerate a wide range of clinical and laboratory-derived qPCR inhibitors. This includes standard laboratory chemicals (SDS, guanidine, and ethanol) and biological sample inhibitors, such as those found in blood samples (hemin, hematin, hemoglobin, heparin, immunoglobulins (IgGs), lactoferrin, sodium citrate), saliva, urine (urea), plant, and environmental samples (humic acid, catechin, quercetin, tannic acid, cellulose, and chlorophyll). The mix has been extensively validated against crude (or diluted) saliva and blood, along with multiple other pure chemical inhibitors.

Features

• Broad-spectrum inhibitor tolerance
• Concentrated 4x mix format, ideal for high-throughput, highly multiplexed assays
• Single and multiplex detection of DNA and RNA
• Contains modified UltraScript^® RTase in a single-tube format
• Includes an advanced RNase inhibitor
• Antibody-mediated hot start technology
• Compatible with all real-time PCR platforms – standard and fast cycling conditions
• Standard and ultra-fast cycling (down to 3 min RT reaction and 1 s denaturation, 3 s annealing/extension for PCR)
• Suitable for use with all probe technologies