Air-Dryable Probe 1-Step Mix

Designed for rapid air-drying, this qPCR mix enables dried assay formats using a standard laboratory oven, offering a simpler, faster, and cheaper alternative to lyophilisation.

The Air-Dryable Probe 1-Step Mix includes a glycerol-free 4x qPCR mix containing hot start Taq polymerase, dNTPs, Mg and a blend of excipients to ensure reliable air-drying, without loss of activity. The mastermix also contains a novel version of UltraScript^® Reverse Transcriptase, modified to increase stability upon drying, and our RiboShield^® RNase inhibitor, so the final blend is suitable both for qPCR detection of DNA and 1-Step RT-qPCR for detection of RNA targets.

Features

• Unbiased, sensitive detection of both DNA and RNA targets, 4 copies per reaction
• Complete 1-step RT-qPCR mix, single tube format
• Easy reaction set up, add only primers & probes before drying
• Rapid air-drying protocol, 80-90 min drying time
• Same high performance before & after drying
• Room temperature storage and shipping of dried reactions
• Increased sample volume input

Key benefits of air-drying

• Reduced volume and mass of reaction mixes leads to cheaper shipping and storage.
• Room temperature stability of the air-dried final gel eliminates the need for cold-chain shipping and storage. This simplifies logistics and reduces overall cost.
• Increased volumes of sample can be used. The sample input volume can equal the final reaction volume. This is perfect for dilute samples (with a low target copy number).
• Air-drying in an oven is a highly accessible method, because there is no requirement for specialist equipment and knowledge, eliminating the need for additional staff or project out-sourcing
• Increased control over the drying process and final product development for small and medium sized IVD kit developers