Clara® Probe 1-Step Mix
RT-qPCRs are no longer a challenge. Get clear and consistent results every time with Clara® Probe 1-Step Mix. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.
This carefully balanced 4x RT-qPCR mix combines maximum sensitivity with ease-of-use for streamlined 1-step probe RT-qPCR workflows. As a result, the mix shows no loss of efficiency in amplifying DNA or RNA and is ideal for users who detect both types of targets without the requirement for separate qPCR reagents.
Features
- Concentrated 4x mix format, ideal for high-throughput, highly multiplexed assays
- Ultra-sensitive detection of RNA & DNA targets
- Early detection of a wide range of template amounts
- Contains modified UltraScript® RTase in a single-tube format
- Advanced RNase inhibitor
- Antibody-mediated hot start technology
- AquaPlex formulation available for improved multiplex performance
- Compatible with all real-time PCR platforms - standard and fast cycling conditions
Multiplex qPCR assay performance may be compromised by certain probe dyes, such as Cy5, which can result in lower fluorescence and delayed amplification. If this is a concern, try our Clara® Probe 1-Step Mix AquaPlex formulation which enables the use of alternative dyes like Texas Red in the Cy5 channel, improving both fluorescence intensity and target detection.