IsoFast® Hot Start Bst Colour Mix

IsoFast^® Hot Start Bst Colour Mix is an isothermal amplification system that enables PCR-free, direct, colour-based readout of DNA targets that is ideal for point-of-care diagnostics and field testing. This 2x mix includes IsoFast^® Hot Start Bst Polymerase which is a recombinant version of the large fragment of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus, Bst) DNA Polymerase. This colourimetric formulation is a preassembled mix allowing direct sample and primer input.

Hot start is important in isothermal amplification techniques because it helps to prevent non-specific amplification and false-positive results. Isothermal amplification methods, such as loop-mediated isothermal amplification (LAMP), rely on specific primers and enzymes to amplify a target sequence at a constant temperature, typically between 60 °C and 65 °C. These techniques are widely used in various applications, including molecular diagnostics and pathogen detection.

Features

• Fast colour readout for positive/negative testing
• AptaLock™ hot start for ultra-sensitive detection of DNA targets
• Rapid polymerisation for faster time to results (as little as 10 mins)
• Detect down to 3 target copies per μL
• Ideal for both cold and room temperature setup
• Improved speed and sensitivity for early target detection
• High activity at a broad range of temperatures from 55-70 °C