IsoFast® Hot Start Bst Colour Mix
IsoFast® Hot Start Bst Colour Mix is a colourimetric isothermal amplification enzyme formulation that combines IsoFast® Hot Start Bst Polymerase with a pH-based dye for rapid positive/negative screening.
Fast colour readout for rapid screening
Easy screening for positives
Convenient 2x mix
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IsoFast® Hot Start Bst Colour Mix is an isothermal amplification system that enables PCR-free, direct, colour-based readout of DNA targets that is ideal for point-of-care diagnostics and field testing. This 2x mix includes IsoFast® Hot Start Bst Polymerase which is a recombinant version of the large fragment of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus, Bst) DNA Polymerase. This colourimetric formulation is a preassembled mix allowing direct sample and primer input.
Hot start is important in isothermal amplification techniques because it helps to prevent non-specific amplification and false-positive results. Isothermal amplification methods, such as loop-mediated isothermal amplification (LAMP), rely on specific primers and enzymes to amplify a target sequence at a constant temperature, typically between 60 °C and 65 °C. These techniques are widely used in various applications, including molecular diagnostics and pathogen detection.
Features
- Fast colour readout for positive/negative testing
- AptaLock™ hot start for ultra-sensitive detection of DNA targets
- Rapid polymerisation for faster time to results (as little as 10 mins)
- Detect down to 3 target copies per μL
- Ideal for both cold and room temperature setup
- Improved speed and sensitivity for early target detection
- High activity at a broad range of temperatures from 55-70 °C
