IsoFast® Hot Start Bst Polymerase
IsoFast® Hot Start Bst Polymerase is the most recent and improved development of isothermal amplification technology, designed to deliver unparalleled specificity and speed in DNA amplification processes. This mix is suitable for applications such as multiple displacement amplification, isothermal amplification, loop mediated isothermal amplification (LAMP), molecular diagnostics and field diagnostics.
Get more sensitive and reliable results
Faster amplification at ambient setup
Faster time to result
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IsoFast® Hot Start Bst Polymerase is a recombinant version of the large fragment of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus, Bst) DNA Polymerase. It is engineered to retain 5’-3’ polymerase activity, but lacks 5’-3’ exonuclease activity. The enzyme’s strong strand displacement capability eliminates the need for denaturation required by standard PCR. Combined with powerful hot start technology, to minimise primer dimer formation and non-specific target amplification, this novel formulation is ideal for non-PCR based amplification.
Features
- AptaLock™ hot start for ultra-sensitive detection of DNA targets
- Rapid polymerisation for faster time to results (as little as 10 mins)
- Detect down to 3 target copies per μL Ideal for both cold and room temperature setup
- Improved speed and sensitivity for early target detection
- High activity at a broad range of temperatures from 55-70
- Available with and without fluorescent dye, and as a 2x ready mix
- Formats available for colourimetric assays
- This product is not suitable for PCR
