Lyo-Ready Probe 1-Step Kit
Lyo-Ready Probe 1-Step Kit is a lyophilisable (freeze-dryable) RT-qPCR kit designed for ultra-sensitive detection of RNA sequences.
Freeze-dryable RT-qPCR mix
Outperforms competitor in multiplex setup
Reliable performance after drying
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This specially formulated kit is ideal for the development of diagnostic assays. It contains a glycerol-free 4x qPCR mix comprising hot start Taq polymerase, dNTPs, Mg and a blend of excipients to provide optimal functionality without loss of activity post lyophilisation. UltraScript® Reverse Transcriptase is provided separately in a highly concentrated 3200x format. This robust and thermostable modified MMLV reverse transcriptase is engineered to enhance cDNA synthesis speed and yield with accurate transcript representation and enables fast and efficient reverse transcription up to 55 °C.
Lyophilisation offers many advantages including ambient storage, extended shelf-life and increased versatility in sample volume. The kit has been optimised to offer the same high performance post-lyophilisation as our standard 1-step RT-qPCR kits.
Features
- Ultra-sensitive detection of RNA sequences
- Offers the same high performance post-lyophilisation as our standard kits
- Includes a glycerol-free 4x qPCR mix
- Pre-blended with excipients for reliable lyophilisation into beads or cakes
- Provided with separate, high-concentration UltraScript RTase (with RNase inhibitor)
- Validated for qualitative detection of SARS-CoV-2 nucleic acid
- Compatible with high-throughput, highly-multiplexed assays
- 4x qPCR mix also available separately for DNA detection only
