MACSQuant® X Flow Cytometer
Reliable high-throughput flow cytometry
MACSQuant X Flow Cytometer
MACSQuant X - Liquid Handling Integration
MACSQuant X - Optical Bench
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Fast acquisition, versatile and easy data analysis.
Natural products screening
We use the cytometer for general purposes and, specifically, to run high throughput screening assays for antibiotic and antifungic activity of natural products. It can run plates of 12 up to 384 wells, and even you can define 12-48 eppendorf tubes acquisition setup, meaning that it's very versatile. The speed of run depends on the target, the cell type, the number of events to acquire and the viscosity of the natural product to test (the washing steps between wells, increase). In normal conditions 96 well plate, acquiring 10000 events per well takes 35 minutes in our hands, with less than 0.11 of carryover. It allows us to run libraries of hundreds of compounds per day. The system is robust, and the technical service is very good, keeping the instrument in its best shape. As analysis software we use Flowlogic, that provide fast plate analysis and easy lead detection. MACSQuant X worth its price.
Review Date: 6 Nov 2019 | Miltenyi Biotec B.V. & Co. KG
Engineered from the ground up as a compact flow cytometer, the MACSQuant® X was made to provide speed and reliability in high-throughput screening. It allows you to get more data, in less time, with less effort. This flow cytometer is perfect for maximizing efficiency, while enjoying the certainty of getting reliable results from every tube, 96- or 384-well plate. The MACSQuant X has been designed to meet high-throughput cell analysis needs for the most challenging large-scale screening settings.
Technology highlight
- Integration with liquid handling systems and plate hotels
- Capable of processing up to 9,200 samples per day (96-well plate within 15 minutes, 384-well plate in under 60 minutes)
- Orbital and needle mixing ensure consistency and accuracy in assay performance and cell counting
- 21 CFR Part 11 compliant software option
Product Specs:
- Quantity: EA
- Dimensions: (WxDxH* *adjustable touch screen) 814x474x427-586.5mm
- Applications: Flow Cytometry
- Excitation:
- 405nm (violet)
- 488nm (blue)
- 640nm (red)
- Detection: FSC, SSC, 8 fluorescence channels
- Regulatory status: For research use only
- Sample format: 1.5/2.0/5.0/15/50 mL tubes or 24/48/96/384-well plates
- Measurement speed: < 15 minutes per 96-well plate (5 µL measurement volume per well) Referred value indicates the average of multiple experiments and can differ for individual sample materials. The measurement speed is determined by measuring the time between the movement of the robotic arm into the first measured well, and its movement out of the last measured well. The measurements were carried out at high flow rate in fast mode
- Maximum event rate: Up to 15.000 events/second
- Sample uptake volume: 1-5000 µL
- Sample carry-over: 0.1 % Referred value indicates the average of multiple experiments and can differ for individual sample materials. For carry-over, full 96-well plates were loaded with 200 μL/well of PBMC suspension at a nominal concentration of 5,000 cells/μL in every other well ("SRC-wells”). Alternating wells are loaded with an equal volume of MACSQuant® Running Buffer ("CO-wells”). The uptake volume was set to 50 μL at medium flowrate. Plate shaking was set to 1200 rpm and a singlet gate was used to determine singlet counts in originating wells as well as in carry-over wells. The carry-over is defined by sum (CO-singletcount)/sum(SRC-singletcount) ×100%. Results for 384-well plates do not differ significantly from those measured in 96-well plates.
- Flow rate: 25-100 µL/min plus automated flow rate to maintain 500, 1000, or 2000 events/second
- Fluorescence performance: 5-decade logarithmic scale (10-2 to 103), displayed in lin, log, or hlog scales
- Compensation: Automated, or manual with 8x8 matrix, during or after acquisition
- Operating system: Embedded operating system
- Software: MACSQuantify, integrated acquisition and analysis software
- Data files: .mqd (proprietary file format), .fcs (2.0, 3.0, 3.1 compatible)
- Components: Build-in PC, 15.6” LCD touchscreen, Robotic uptake unit for sample acquisition, Orbital shaker
- Accessories:
- MACS® Chill Racks (5, 15, 50, 96)
- Universal Reagent Rack (130-115-722)
- MACSQuant® Buffer Supply Station (130-101-841)
