NGSBIO Library Quant Kit for Illumina

The NGSBIO Library Quant Kit contains all the components required for accurate and sensitive quantification of libraries prepared for Illumina NGS systems. The kit uses qPCR to specifically quantify adapter-ligated DNA molecules, ensuring optimal cluster densities for improved sequencing efficiency and quality of data.

qPCR is considered the best method for quantifying NGS libraries as it only measures adapter-bound molecules that can be used as templates for library amplification and cluster generation. The NGSBIO Library Quant Kit enables highly accurate quantification crucial for optimal cluster densities and greater sequencing efficiency. For easy and accurate data analysis we recommend using our NGSBIO Library Quantification Tool available on our website. Library quantification can be used at any time after adapter ligation and should always be used prior to cluster generation. The DNA standards supplied are precisely measured and ready-to-use, covering 5 orders of magnitude from 2 pM to 0.2 fM. The kit is suitable for quantification of even low concentration libraries including libraries constructed without a PCR amplification step.

Features

• Uses qPCR to accurately and rapidly quantify a library prior to sequencing
• Gives consistent library quantification across a wide range of sample types, concentrations, fragment sizes and GC content
• Uses a single extension time for all libraries
• Allows specific quantification of only DNA molecules that can be sequenced by NGS
• Uses antibody-mediated hot start technology to ensure all reactions start simultaneously
• Compatible with all Illumina instruments and qPCR platforms
• Suitable for manual and automated workflows
• Easily calculate library concentration with the online NGSBIO Library Quantification Tool