PCRBIO HiFi Polymerase
PCRBIO HiFi Polymerase is our original high-fidelity enzyme, derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. Several proprietary point mutations allow for significantly improved performance when compared with its native form. Together with advanced buffer chemistry this enzyme brings robust performance to the world of high fidelity PCR.
Enhanced processivity in PCR
Stable for 28 days at 4 °C, 25 °C and 37 °C
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PCRBIO HiFi Polymerase uses the latest developments in DNA polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system give superior PCR performance on complex templates such has mammalian genomic DNA. Enhanced DNA binding allows for improved processivity, increasing yield and shortening cycling times (35 cycles of a 5 kb amplicon in under 1.5 hours).
PCRBIO HiFi Polymerase performs consistently well on a broad range of templates including both GC and AT rich.
Features
- 50x higher fidelity than Taq DNA polymerase
- Increased PCR success rates with longer amplicons
- Advanced buffer chemistry including Mg and dNTPs
- High yields under standard and fast PCR conditions
- Efficient and specific amplification from complex templates including GC rich and AT rich sequences
