PCRBIO Ultra Mix
PCRBIO Ultra Mix is a convenient 2x ready mix that has been engineered for the amplification of extremely difficult templates. Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT-rich, low in abundance or contains PCR inhibitors.
A convenient ready mix for difficult PCR
High yield at a range of fragment lengths
Amplifies 69% and 71% GC fragments
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PCRBIO Ultra Mix is a highly robust 2x ready mix, designed for efficient and reliable amplification of challenging and complex targets, even under difficult conditions such as the presence of inhibitors. The enzyme and buffer system have been developed to give superior PCR performance and higher success rates on a broad range of templates, including complex genomic DNA and targets with a high GC content (up to 80% GC).
PCRBIO Ultra Mix exhibits a high tolerance to PCR inhibitors making it the ideal choice for colony and crude sample PCR. PCR products generated are A-tailed and may be cloned into TA cloning vectors. The mix includes an optimised buffer containing dNTPs and Mg. Our antibody-mediated hot start formulation prevents the formation of primer dimers and non-specific products, enabling specific and sensitive amplification from low copy number target sequences.
Features
- Ready-to-use 2x mix
- Increased PCR success rates with difficult templates
- Exhibits high tolerance to PCR inhibitors
- Efficient specific amplification from complex templates including GC-rich sequences (up to 80%)
- Antibody-mediated hot start for unrivaled detection of low copy number templates
- Advanced buffer chemistry including Mg and dNTPs
- High yields under standard and fast PCR conditions
- 3 fold higher fidelity than Taq
