PCRBIO Ultra Mix Red

PCRBIO Ultra Mix is a highly robust 2x ready mix, designed for efficient and reliable amplification of challenging and complex targets, even under difficult conditions such as the presence of inhibitors. The mix includes a red dye for direct gel loading. The enzyme and buffer system have been developed to give superior PCR performance and higher success rates on a broad range of templates, including complex genomic DNA and targets with a high GC content (up to 80% GC).

PCRBIO Ultra Mix exhibits a high tolerance to PCR inhibitors making it the ideal choice for colony and crude sample PCR. PCR products generated are A-tailed and may be cloned into TA cloning vectors. The mix includes an optimised buffer containing dNTPs and Mg. Our antibody-mediated hot start formulation prevents the formation of primer dimers and non-specific products, enabling specific and sensitive amplification from low copy number target sequences.

Features

• Ready-to-use 2x mix with red dye for direct gel loading
• Increased PCR success rates with difficult templates
• Exhibits high tolerance to PCR inhibitors
• Efficient specific amplification from complex templates including GC-rich sequences (up to 80%)
• Antibody-mediated hot start for unrivaled detection of low copy number templates
• Advanced buffer chemistry including Mg and dNTPs
• High yields under standard and fast PCR conditions
• 3 fold higher fidelity than Taq