UltraScript® cDNA Synthesis Kit
UltraScript® cDNA Synthesis Kit is an easy-to-use 2-tube system specifically developed to generate cDNA for use in real-time PCR. The kit provides unbiased, efficient and sensitive cDNA synthesis over a broad range of RNA template concentrations.
Easy-to-use 2 tube system for cDNA synthesis
Unbiased representation of mRNA ends
Broad dynamic range of reverse transcription
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UltraScript® cDNA Synthesis Kit (formerly qPCRBIO cDNA Synthesis Kit) includes optimised concentrations of random hexamers and anchored oligo(dT) specifically for cDNA generation for use in real-time PCR experiments. This eliminates the need for user optimisation of the critical factors such as priming strategy, reverse transcriptase, buffer and enhancers, and gives unbiased, efficient and sensitive cDNA synthesis every time.
The kit uses UltraScript® Reverse Transcriptase which is a thermostable and extremely active modified MMLV reverse transcriptase. The reaction temperature can be increased up to 55 °C providing higher specificity and efficient transcription of GC-rich RNA regions with a high secondary structure. The enzyme is blended with an advanced RNase inhibitor preventing degradation of RNA by contaminating RNase. UltraScript® Reverse Transcriptase is not inhibited by ribosomal and transfer RNAs, making total RNA an ideal substrate.
Features
- Unbiased representation of 5’ and 3’ mRNA transcript ends
- Reduced RNase H activity
- Thermostable reverse transcriptase 45 °C to 55 °C
- Advanced RNase inhibitor
- High cDNA yields from as little as 4 pg total RNA
- Accurate reverse transcription of GC-rich templates
- Sensitive detection of low copy number transcripts
- Advanced buffer chemistry including Mg and dNTPs
- Both premixed or separate oligo cDNA synthesis kits available
