VeriFi® Hot Start Mix

VeriFi^® Hot Start Mix is as a convenient 2x ready mix containing all reaction components apart from primers and template. The mix is powered by VeriFi^® Hot Start Polymerase, a single enzyme derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. Proprietary mutations improve DNA binding and increase processivity when compared to its native form, resulting in shorter extension times, higher yields and the ability to amplify longer and more difficult targets. VeriFi^® Hot Start Polymerase is able to amplify eukaryotic genomic templates in excess of 17.5 kb, and longer for simpler DNA templates.

PCRBIO’s innovative AptaLock™ technology uses a proprietary aptamer-like molecule that reversibly inhibits activity below 40 °C, preventing primer dimer formation and non-specific amplification to maximise the sensitivity and specificity of your PCR. This feature makes VeriFi^® Hot Start Mix highly suitable for multiplexing and enables reactions to be set up at room temperature. The reaction mix is stable both before and after the PCR run for up to 24 hours, meaning greater ease of use for high throughput assays.

The enhanced accuracy of VeriFi^® Hot Start Polymerase gives extremely low error rates and fidelity that is approximately 100 times higher than Taq DNA polymerase. The enzyme is ideal for applications where superior accuracy is required, such as cloning, site-directed mutagenesis and sequencing.

Features

• AptaLock™ hot start technology for maximised sensitivity and specificity
• Greater success with long and/or GC or AT-rich templates (17.5 kb and over)
• High temperature cycling – up to 100 °C denaturation to better separate GC-rich sequences
• 100x higher fidelity than Taq DNA polymerase
• Room temperature setup
• Reaction mix stability for up to 24 hours both before and after PCR run
  Generates blunt-end PCR products