VeriFi® Library Amplification Mix
VeriFi® Library Amplification Mix is ideal for NGS library amplification workflows and challenging PCRs. Combining a powerful and robust proofreading enzyme, greatly reduced GC-dependent bias, and AptaLock™ hot start technology, this mix enables precise PCR, regardless the target you are sequencing.
2x ready-mix for library amplification
Higher unique reads with VeriFi
VeriFi (A) outperforms across a broad GC range
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VeriFi® Library Amplification Mix was created as a tool for researchers conducting next generation sequencing (NGS) studies.
VeriFi® is a superior proofreading Pfu polymerase in a specially formulated 2x PCR ready mix designed for NGS library amplification with reduced GC bias. This cutting-edge PCR mix offers market leading performance enabling the acquisition of superior quality datasets with a higher number of unique reads.
Novel buffer composition makes VeriFi® Library Amplification Mix ideal for complex templates. We challenged this enzyme in PCRs on numerous difficult templates, demonstrating minimal bias during amplification at extremes of GC content ranging from 30% – 70%. With consistently better performance than all major competitors, these results ensure cutting-edge performance of the mix regardless of the target sequence, to give you confidence when undertaking costly and laborious NGS experiments.
Features
- Low GC bias, ideal for high GC/AT targets
- More unique reads per NGS dataset for superior data quality
- AptaLock® hot start technology for maximum sensitivity and specificity
- 100x higher fidelity than Taq DNA polymerase
- Room temperature setup 2x ready mix for minimal pipetting
