VeriFi® Mix
VeriFi® Mix is a versatile and robust high fidelity 2x ready-mix engineered for all PCR applications where greater sequence accuracy is required. Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets.
A ready-mix with a high fidelity enzyme
Greater sensitivity and specificity
Versatility across amplicon lengths
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VeriFi® Mix includes VeriFi® Polymerase which is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. The enzyme is engineered with proprietary mutations that significantly increase processivity, resulting in shorter extension times (10-30 s/kb), higher yields and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5 kb.
The high accuracy and enhanced 3’-5’ exonuclease activity of VeriFi® Polymerase result in fidelity that is approximately 100 times higher than Taq DNA polymerase. The mix is ideally suited to applications where greater accuracy is required, such as cloning, site-directed mutagenesis and sequencing. PCR products generated with this range of products are blunt ended.
This 2x mix contains all reaction components apart from primers and template.
Features
- High temperature cycling – up to 100 °C denaturation to better separate GC-rich templates
- Increased PCR success rates with complex genomic templates (17.5 kb and over)
- High yields under standard and fast PCR conditions (10-30 s/kb)
- Efficient and specific amplification from challenging templates including GC and AT-rich sequences
- 100x higher fidelity than Taq DNA polymerase
- Generates blunt-end PCR products
