VeriFi® Mix Red
VeriFi® Mix Red is a versatile and robust high fidelity 2x ready-mix engineered for all PCR applications where greater sequence accuracy is required. Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets. The mix includes a convenient red dye for direct gel loading.
A ready-mix with easy to see red dye
Greater sensitivity and specificity
Versatility across amplicon lengths
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5* would recommend
Molecular Biology
Fantastic polymerase with dye, high quality PCR products, for use in gibson assembly, gene knockouts and sequencing. Easy to use, add to everything else. Fire away. Ready to load into a gel immediately. Absolute dream. 5* would recommend to friends and colleagues.
Review Date: 5 Nov 2024 | PCR Biosystems
VeriFi® Mix Red includes VeriFi® Polymerase which is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. The enzyme is engineered with proprietary mutations that significantly increase processivity, resulting in shorter extension times (10-30 s/kb), higher yields and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5 kb.
The high accuracy and enhanced 3’-5’ exonuclease activity of VeriFi® Polymerase result in fidelity that is approximately 100 times higher than Taq DNA polymerase. The mix is ideally suited to applications where greater accuracy is required, such as cloning, site-directed mutagenesis and sequencing. PCR products generated with this range of products are blunt ended.
This 2x red mix contains all reaction components apart from primers and template, and a red dye suitable for direct loading and tracking during agarose gel electrophoresis.
Features
- High temperature cycling – up to 100 °C denaturation to better separate GC-rich templates
- Increased PCR success rates with complex genomic templates (17.5 kb and over)
- High yields under standard and fast PCR conditions (10-30 s/kb)
- Efficient and specific amplification from challenging templates including GC and AT-rich sequences
- 100x higher fidelity than Taq DNA polymerase
- Generates blunt-end PCR products
