VeriFi® Polymerase
VeriFi® Polymerase is a versatile and robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required. Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets.
A high fidelity enzyme for greater sequence accuracy
Greater sensitivity and specificity
Versatility across amplicon lengths
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Great for high GC, large DNA fragment PCRs
PCR
This polymerase is my go to for problematic DNA fragments for cloning/assembly purposes. Tested on high GC (70-80%), large (5kb+) DNA fragments from Streptomyces. I tested HS VeriFi against 5 or so competitors and this came out on top. Advise running with at 98C for high GC - it does make a difference in my experience.
Review Date: 13 May 2024 | PCR Biosystems
VeriFi® Polymerase is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. The enzyme is engineered with proprietary mutations that significantly increase processivity, resulting in shorter extension times (10-30 s/kb), higher yields and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5 kb.
The high accuracy and enhanced 3’-5’ exonuclease activity of VeriFi® Polymerase result in fidelity that is approximately 100 times higher than Taq DNA polymerase. The enzyme is ideally suited to applications where greater accuracy is required, such as cloning, site-directed mutagenesis and sequencing. PCR products generated with this range of products are blunt ended.
VeriFi® Polymerase enables high fidelity PCR of a wide range of targets and fragment sizes with minimal or no optimisation required. The enzyme is provided with an advanced buffer system including both dNTPs and Mg. For the most difficult templates, such as highly GC-rich sequences or those with complex secondary structures, users have the option of adding the VeriMax Enhancer to the reaction mix for improved PCR performance.
Features
- High temperature cycling – up to 100 °C denaturation to better separate GC-rich templates
- Increased PCR success rates with complex genomic templates (17.5 kb and over)
- High yields under standard and fast PCR conditions (10-30 s/kb)
- Efficient and specific amplification from challenging templates including GC and AT-rich sequences
- 100x higher fidelity than Taq DNA polymerase
- Generates blunt-end PCR products
