Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
KAPA Stranded mRNA-Seq Kits includes all the enzymes and buffers required for cDNA library preparation for Illumina Next-Generation Sequencing, utilizing 100 ng – 4 µg of total RNA. KAPA mRNA Capture Beads are included for isolation of poly(A)-tailed RNA. Kits provides precise measurement of strand orientation (>99%), uniform coverage, and high-confidence mapping of alternate transcripts, and are optimized for the improved…
The gold standard for NGS library amplification.
KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of NGS libraries prior to pooling for capture or flow cell amplification. Kits contain KAPA SYBR® FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR. The engineered KAPA SYBR FAST DNA Polymerase contained in the Master Mix amplifies GC- and AT-rich DNA fragments of…
KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent rea…
KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting dramatic improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kit is optimized for rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination with a convenient qRT-PCR protocol. KAPA SYBR FAST One-Step qRT-PCR Kits contain M-MuLV r…
High Resolution Melt (HRM) analysis is a technique for fast, high-throughput post-PCR analysis of genetic mutations or variance in nucleic acid sequences. The KAPA HRM FAST Master Mix contains a novel DNA polymerase and buffer system, and EvaGreen® saturating dye optimized for maximum discrimination between sequence variants. Features: Maximize differences in sequence variants Engineered to maximize differe…
KAPA HiFi DNA Polymerase provides high fidelity next-generation sequencing library amplification with the highest yield, lowest bias, and most uniform sequence coverage available. These characteristics are retained in KAPA HiFi Uracil+ DNA Polymerase, a modified version of KAPA HiFi engineered to tolerate uracil residues. The new enzyme is particularly well suited for applications employing bisulfite DNA conversion, which t…
KAPA2G Fast Multiplex PCR Kits contain a second-generation (2G) enzyme derived through a process of directed evolution. KAPA2G FAST HotStart DNA Polymerase is an antibody-mediated hot start formulation engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq DNA polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes,…
KAPA3G Plant PCR Kits are designed for PCR of plant-derived DNA, using either purified DNA or DNA prepared by crude extraction methods (crude sample PCR). In addition, the KAPA3G Plant PCR Kit can be used to amplify DNA from plant material added directly to the PCR (direct PCR). The KAPA3G Plant PCR Kit contains KAPA3G DNA Polymerase, a novel, third-generation (3G) enzyme which was engineered via a process of directed evolu…
KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets. Features: Reduce cycling times up to 75% Extension times as low as 1 sec per kilobase Broad c…
