Products & Reviews

NEBNext® reagents are a series of highly pure reagents that facilitate library preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Available in sets, master mixes and modules, these robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value. Advantages of NEBNext Reagents for RNA Library Pr…

Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid, roo…

The NEBNext® DNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expressi…

he NEBNext® Ultra™ DNA Library Prep Kit for Illumina contains enzymes and buffers that are ideal to convert a small amount of DNA input into indexed libraries for next-generation sequencing on the Illumina platform (Illumina, Inc). The workflow of NEBNext Ultra DNA Library Prep Kit for Illumina is very user-friendly and fast with minimal hands-on time.NEBNext® reagents are a series of highly pure reagents that facilitate samp…

NEBNext® dsDNA Fragmentase® generates dsDNA breaks in a time-dependent manner to yield 100–800 bp DNA fragments depending on reaction time. NEBNext dsDNA Fragmentase contains two enzymes, one randomly generates nicks on dsDNA and the other recognizes the nicked site and cuts the opposite DNA strand across from the nick, producing dsDNA breaks. The resulting DNA fragments contain short overhangs, 5´-phosphates, and 3´-hydroxyl…

The NEBNext® mRNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents…

Rapid, sensitive and precise dye-based qPCR detection and quantitation of target DNA and cDNA sequences

The LunaScript® RT SuperMix Kit is optimized for cDNA synthesis in a two-step RT-qPCR workflow.

The unique workflow of the NEBNext Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.  

Rapid, sensitive, and precise probe-based qPCR detection and quantitation of target RNA targets.

The NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® contains enzymes and buffers that are ideally suited for cDNA library preparation for next-generation sequencing. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents included within these master mi…

The NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina offers an amplification-free workflow for DNA-seq based on the streamlined and reliable Ultra II FS workflow. Starting with as little as 50 ng of DNA, get the high-quality, high-yield libraries that you need without PCR bias.

The NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents in…

The NEBNext® DNA Library Prep Master Mix Set for 454™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of single stranded DNA for use in high density hybridization arrays or for genomic subtraction hybridization methods. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation o…

The NEBNext® DNA Library Prep Set for SOLiD™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library cons…

The NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing on an Ion Torrent PGM™. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library…

The NEBNext® Fast DNA Library Prep Set for Ion Torrent™ contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing on a Ion Torrent PGM™. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. T…

The NEBNext Quick DNA Library Prep Master Mix Set for 454 contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expressio…

New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction.NEBNext® dsDNA Fragmentase Reaction Buffer Components: 20mM Tris…

The NEBNext mRNA Library Prep Master Mix Set for 454 contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents includ…

The NEBNext® Ultra™ RNA Library Prep Kit for Illumina contains enzymes and buffers that are ideally suited for cDNA library preparation for next-generation sequencing. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents included within these master mix sets must p…

The NEBNext Small RNA Library Prep Set for SOLiD™ contains adaptors, primers, enzymes and buffers that are ideal to convert small RNA into cDNA libraries for next-generation sequencing on the SOLiD platform. NEBNext Small RNA Library Prep Set for Solid is multiplex compatible. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next genera…