Life Sciences Products & Reviews

Polyclonal secondary antibodies raised in goat, rabbit or donkey, immunoaffinity-purified using corresponding immobilized antigens and conjugated to horseradish peroxidase (HRP) enzyme.   The Anti-Human IgG (H+L), HRP Conjugate, Anti-Mouse IgG (H+L), HRP Conjugate and Anti-Rabbit IgG (H+L), HRP Conjugate antibodies bind to both heavy and light chains for all IgG subclasses. As with all antibodies, in certain applications…

Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues.   The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in addition…

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes).   Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification pri…

The ReliaPrep™ RNA Miniprep Systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes.   The proprietary column/binding matrix can efficiently capture RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15µl). Using this membrane-based purification system, from 100 to 5 × 106 cultured cells or fro…

The Wizard® SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100bp to 10kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests.   Up to 95% recovery is achieved depending upon the DNA fragment size. PCR products are commonly purified to remove excess nucleotides and primers. This membrane-based system, wh…

The PureYield™ Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA.   The system provides a rapid method for purification of up to 15μg of plasmid DNA from 600μl to 3ml of bacteria culture. Plasmid DNA can be purified in as little as 10 minutes. The PureYield™ Plasmid Miniprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxi…

ViaFect™ Transfection Reagent allows high-efficiency transfection of a wide range of cell types, including iPS Cells, without compromising cell viability.   ViaFect™ Transfection Reagent enables the design of biologically relevant assays in workhorse adherent cell models, suspension cells important for studying cell signaling, and even differentiated cell lines derived from stem cells. ViaFect™ Transfection Reagent offer…

This ready-to-use multimode detection system includes: high-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities.   The GloMax® Discover System is a high-performance multimode detection instrument developed with Promega reagent chemistries to provide a simple means of detecting advanced chemistries. This instrument provides…

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase.   The luminescent signal positively correlates with kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary scre…

The Maxwell® 16 LEV simplyRNA Kits are for use with the Maxwell® 16 Instrument configured with the LEV High Strength Magnetic Rod and Plunger Bar Adaptor.   This RNA purification procedure is a simple method with minimal lysate handling before automated purification on the Maxwell® 16 Instrument. The low elution volume is used to generate concentrated high-quality RNA suitable for use in downstream applications such…