Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations. The high RT efficiency of SuperScript III RT and the optimized formulation allow the mast…
Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates. It includes an enzyme blend of Platinum Taq DNA Polymerase and a proofreading enzyme, plus AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases. With AccuPrime Taq DNA Polymerase, High Fidelity, you'll get: • The…
Components : Tks Gflex DNA Polymerase 250 U 2 X Gflex PCR Buffer (Mg2+, dNTP plus) 1 ml x 5 Mg2+ conc. (2 X) 2 mM dNTP conc. (2 X) ea. 400 μM
KAPA2G Fast PCR Kits contain a second-generation (2G) DNA polymerase enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast PCR Kit provides higher yields and sensitivity than competitor enzymes across a broad range of targets.
Thermo Scientific Tubes and Domed Caps, Strips of 8 are designed for PCR applications, and are suitable for 0.2 mL thermal cycler blocks and 96-well V-bottom thermal cyclers. The 8-strip tubes can be divided into individual tubes.
Concentrated clean up with small elution volumes from PCR reaction mixtures and agarose gels
FastStart Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. This enzyme delivers superior results thanks to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is inactive at temperatures below 75°C, but is activated by a 2- to 4-minute incubation step at 95°C. PCR Hot Start PCR RT-PCR Benefits Insist on higher specificity, sensitivity…
Upgrade from the Expand Long Template PCR System and choose the new Expand Long Range dNTPack for consistent amplification of long PCR products. Generate PCR products of 5 to 25 kb from genomic DNA with this next-generation system from the company that pioneered long-template PCR. This specially designed PCR system combines a single buffer set for all product sizes, ultrapure PCR-grade nucleotide mix, and an optimized blend of…
The Advantage® RT-for-PCR Kit allows first-strand cDNA synthesis from either total or poly A+ RNA. With this kit, you can obtain sufficient quantities of cDNA for PCR from nanogram quantities of any RNA. The Advantage® RT-for- PCR Kit offers several advantages: Longer oligo(dT) primers provide more effective cDNA priming. Recombinant-derived RNase inhibitor minimizes RNA degradation. Each kit contains everything you ne…
Piko™ Thermal Cyclers - high thermal performance in a tiny footprint. Piko, inspired by the Italian word “piccolo,” meaning tiny, aptly describes Finnzymes’ new PCR system. At the heart of the Piko Thermal Cycler lies our unique heat pump. Designed to deliver bridled power, it results in industry-leading thermal uniformity, accuracy, ramp rates and settling times. This not only allows for PCR protocols in as little as 10 minut…
Applied Biosystems’ latest entry-level real-time PCR instrument, the StepOne™ Real-Time PCR System, makes it simple and easy to get high-quality real-time PCR results. This remarkably simple real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers new to real-time PCR.
Obtain superior sensitivity and reproducibility without compromising any performance parameters in your real-time, quantitative PCR experiments. Power SYBR® Green PCR Master Mix has a new look! The new pouch design eliminates excess packaging materials, resulting in 72% less material than the carton with platform & label. The master mix inside the pouch is unchanged.
TaqMan® MicroRNA Arrays provide all the advantages of TaqMan® MicroRNA Assays in a convenient, pre-configured micro fluidic card minimizing experimental variability and effort required to run 384 TaqMan MicroRNA Assays in parallel. Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and simplify the TaqMan MicroRNA Array. When sensitivity is of th…
The MICROLAB® NIMBUS is Hamilton Company's newest automated multi-channel pipetting platform, offering a high density deck layout in a body small enough to fit inside a laminar flow hood. The system utilizes the same highly accurate 96-channel pipetting head as Hamilton's STAR instruments, yet is designed for smaller throughput applications. The NIMBUS is ideal for: • Fast plate replications • Row-wise and column-wide serial d…
High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. The MeltDoctor™ HRM Master Mix contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed…
The new MultiGene™ OptiMax replaces our successful and reliable MultiGene™ Gradient thermal cycler and brings new speed and advanced features to our Labnet Thermal Cycler line providing premium performance at an affordable price. • Unique Flexible Programming with FlexTemp™ technology • Fast ramp rates 5oC heating / 3.5oC cooling • Simple user interface • No condensation after overnight cooling at 4°C • 3 Year Warranty The Mul…
The TProfessional Basic Thermocycler features a gold anodized 96 well block or an anodized 96 well XL block (NEW!!!) for extra large volumes up to 100µl. The gold anodized 96 well block is designed for standard applications whereas the XL block is especially developed for special protocols where large volumes are required like emulsion PCR for new generation sequencing. Both block types offer an excellent temperature uniformit…
The SYBR® Green PCR Master Mix, optimized for real-time PCR analysis, conveniently combines SYBR Green 1 Dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer components in easy-to-use premix vials. SYBR® Green Master Mix has a new look! The new pouch design eliminates excess packaging materials, resulting in 72% less material than the carton with platform & label. The master mix inside…
The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. Applied Biosystems’ latest introduction to real-time PCR, the StepOnePlus™ Real-Time PCR System, makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful…
Exiqon miRCURY LNA™ First-strand cDNA Kit First-strand cDNA synthesis reagents for high performance microRNA real-time PCR using the miRCURY LNA™ microRNA PCR system. Features of the Exiqon miRCURY LNA™ First-strand cDNA Kit: • Optimized for fast, easy and reliable first-strand cDNA synthesis using our miRCURY LNA™ microRNA PCR system • Includes a thermostable and highly reliable reverse transcriptase The miRCURY LNA™ Fir…
The LightCycler® 2.0 Instrument allows you to monitor amplification of 32 PCR products simultaneously, in real-time and online, with six different detection channels. The LightCycler® Software 4.1 collects fluorescence data from all six channels at each cycle and displays it immediately. This enables you to terminate PCR runs early if signal levels are adequate, or prolong them when signals are too low. Amplification occurs in…
The GeneMorph II Random Mutagenesis Kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.
Application The MagNA Pure LC Cooling Block, 96-well PCR Plate, is used to hold 0.2 ml PCR plates, 0.2 ml 8-strip vials, or 0.2 ml single vials for the most commonly used PCR block cyclers. It is placed on the stage of the MagNA Pure LC Instrument prior to automatic filling by the MagNA Pure LC Instrument. The stage of the MagNA Pure LC Instrument includes a cooling area that is designed for various post-elution pipetting…
Thermo Scientific Arktik Thermal Cycler is suitable for a wide range of applications from individual reactions to high-throughput projects. It offers flexibility with three different interchangeable blocks: 96-well block, 384-well block, and a dual block with two 48-well units. A broad temperature gradient in 96 and 384 blocks simplifies temperature optimization. Ease-of-use is the key feature of the Arktik Thermal Cycler’s us…
The PrimeScript RT-PCR Kit is a two-step RT-PCR kit featuring PrimeScript RTase, an RNase H Minus reverse transcriptase enzyme that offers robust reverse transcription of virtually any RNA template. This RT-PCR kit is conveniently configured to include all reagents needed for two-step endpoint RT-PCR, including primers, PrimeScript RTase, and PCR polymerase enzyme. PrimeScript RTase is an MMLV Reverse Transcriptase and enable…
