PCR and Thermal Cycling Products & Reviews

Invitrogen™ Platinum™ SuperFi™ PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle). The…

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations. The high RT efficiency of SuperScript III RT and the optimized formulation allow the mast…

Components : Tks Gflex DNA Polymerase 250 U 2 X Gflex PCR Buffer (Mg2+, dNTP plus) 1 ml x 5 Mg2+ conc. (2 X) 2 mM dNTP conc. (2 X) ea. 400 μM

Nexar™ is a modular inline liquid handling and assay processing system offering a highly scalable solution for PCR-based genotyping.

KAPA2G Fast PCR Kits contain a second-generation (2G) DNA polymerase enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast PCR Kit provides higher yields and sensitivity than competitor enzymes across a broad range of targets.

Thermo Scientific Tubes and Domed Caps, Strips of 8 are designed for PCR applications, and are suitable for 0.2 mL thermal cycler blocks and 96-well V-bottom thermal cyclers. The 8-strip tubes can be divided into individual tubes.

Concentrated clean up with small elution volumes from PCR reaction mixtures and agarose gels

The FailSafe™ PCR System provides dependable, consistent high-fidelity PCR results for every DNA template, regardless of its source or sequence. The FailSafe PCR System will faithfully amplify your template every time. The system includes the FailSafe™ PCR Enzyme Mix and a meticulously tested set of 12 FailSafe™ PCR “PreMixes” that cover a matrix of enzyme-specific PCR conditions that are optimal for amplifying different se…

FastStart Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. This enzyme delivers superior results thanks to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is inactive at temperatures below 75°C, but is activated by a 2- to 4-minute incubation step at 95°C. PCR Hot Start PCR RT-PCR Benefits Insist on higher specificity, sensitivity…

Upgrade from the Expand Long Template PCR System and choose the new Expand Long Range dNTPack for consistent amplification of long PCR products. Generate PCR products of 5 to 25 kb from genomic DNA with this next-generation system from the company that pioneered long-template PCR. This specially designed PCR system combines a single buffer set for all product sizes, ultrapure PCR-grade nucleotide mix, and an optimized blend of…

The Advantage® RT-for-PCR Kit allows first-strand cDNA synthesis from either total or poly A+ RNA. With this kit, you can obtain sufficient quantities of cDNA for PCR from nanogram quantities of any RNA. The Advantage® RT-for- PCR Kit offers several advantages: Longer oligo(dT) primers provide more effective cDNA priming. Recombinant-derived RNase inhibitor minimizes RNA degradation. Each kit contains everything you ne…

Piko™ Thermal Cyclers - high thermal performance in a tiny footprint. Piko, inspired by the Italian word “piccolo,” meaning tiny, aptly describes Finnzymes’ new PCR system. At the heart of the Piko Thermal Cycler lies our unique heat pump. Designed to deliver bridled power, it results in industry-leading thermal uniformity, accuracy, ramp rates and settling times. This not only allows for PCR protocols in as little as 10 minut…

Applied Biosystems’ latest entry-level real-time PCR instrument, the StepOne™ Real-Time PCR System, makes it simple and easy to get high-quality real-time PCR results. This remarkably simple real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers new to real-time PCR.

Obtain superior sensitivity and reproducibility without compromising any performance parameters in your real-time, quantitative PCR experiments. Power SYBR® Green PCR Master Mix has a new look! The new pouch design eliminates excess packaging materials, resulting in 72% less material than the carton with platform & label. The master mix inside the pouch is unchanged.

TaqMan®  MicroRNA Arrays provide all the advantages of TaqMan®  MicroRNA Assays in a convenient, pre-configured micro fluidic card minimizing experimental variability and effort required to run 384 TaqMan MicroRNA Assays in parallel. Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and simplify the TaqMan MicroRNA Array. When sensitivity is of th…

High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. The MeltDoctor™ HRM Master Mix contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed…

The new MultiGene™ OptiMax replaces our successful and reliable MultiGene™ Gradient thermal cycler and brings new speed and advanced features to our Labnet Thermal Cycler line providing premium performance at an affordable price. • Unique Flexible Programming with FlexTemp™ technology • Fast ramp rates 5oC heating / 3.5oC cooling • Simple user interface • No condensation after overnight cooling at 4°C • 3 Year Warranty The Mul…

The TProfessional Basic Thermocycler features a gold anodized 96 well block or an anodized 96 well XL block (NEW!!!) for extra large volumes up to 100µl. The gold anodized 96 well block is designed for standard applications whereas the XL block is especially developed for special protocols where large volumes are required like emulsion PCR for new generation sequencing. Both block types offer an excellent temperature uniformit…

The SYBR® Green PCR Master Mix, optimized for real-time PCR analysis, conveniently combines SYBR Green 1 Dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer components in easy-to-use premix vials. SYBR® Green Master Mix has a new look! The new pouch design eliminates excess packaging materials, resulting in 72% less material than the carton with platform & label. The master mix inside…

The StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. Applied Biosystems’ latest introduction to real-time PCR, the StepOnePlus™ Real-Time PCR System, makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful…

Exiqon miRCURY LNA™ First-strand cDNA Kit First-strand cDNA synthesis reagents for high performance microRNA real-time PCR using the miRCURY LNA™ microRNA PCR system. Features of the Exiqon miRCURY LNA™ First-strand cDNA Kit: • Optimized for fast, easy and reliable first-strand cDNA synthesis using our miRCURY LNA™ microRNA PCR system • Includes a thermostable and highly reliable reverse transcriptase The miRCURY LNA™ Fir…

GoTaq® qPCR Master Mix is a new reagent system for use in real-time quantitative PCR (qPCR). The system contains BRYT™ Green, a new proprietary fluorescent DNA binding dye that exhibits greater fluorescence enhancement upon binding to double-stranded DNA than SYBR® Green I. Combined with the GoTaq® Hot Start Polymerase, optimized buffer and proprietary dye, GoTaq® qPCR Master Mix provides robust real-time PCR with increased re…

The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibit…

The LightCycler® 2.0 Instrument allows you to monitor amplification of 32 PCR products simultaneously, in real-time and online, with six different detection channels. The LightCycler® Software 4.1 collects fluorescence data from all six channels at each cycle and displays it immediately. This enables you to terminate PCR runs early if signal levels are adequate, or prolong them when signals are too low. Amplification occurs in…